{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T08:42:35Z","timestamp":1790412155522,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1993,3,1]],"date-time":"1993-03-01T00:00:00Z","timestamp":730944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1993,3]]},"abstract":"<jats:p>We have performed a set of independent studies on the effects of the circulating pancreatic polypeptide, amylin, on rat osteoclast function, in vitro. Time\u2010lapse video observations, measuring cell protrusions and retraction, showed that 250 nmol l\u20101 amylin or 250 nmol l\u20101 beta\u2010calcitonin gene\u2010related peptide (beta\u2010CGRP) inhibited osteoclast motility (quiescence or Q effect). Both amylin and beta\u2010CGRP produced inhibitory responses with a significant first\u2010order regression over time (half\u2010times, 19 and 28 min respectively). In contrast, 250 nmol l\u20101 amylin or 250 nmol l\u20101 beta\u2010CGRP produced no change of osteoclast spread area, whilst 300 pmol l\u20101 calcitonin (CT) application resulted in cell retraction (R effect). Forskolin (10 mumol l\u20101) mimicked amylin and CGRP in inhibiting osteoclast motility (half\u2010time, 8.6 min), and similarly lacked an effect on cell spread area. Neither amylin nor beta\u2010CGRP (62.5\u20131250 nmol l\u20101) elevated cytosolic free calcium levels ([Ca2+]i) in single osteoclasts whilst 300 pmol l\u20101 salmon calcitonin (sCT) produced a rapid phasic elevation of [Ca2+]i, confirming previous results with asusuberic (1\u20107) eel calcitonin. The osteoclast\u2010bone resorption assay revealed the following potency difference in direct comparison of the area of resorption per bone slice: beta\u2010CGRP\/amylin, 0.1; sCT\/amylin, 800 and human CT\/amylin, 12. The potency of deamidated amylin approached that of beta\u2010CGRP. Assay precision ranged between 0.3 and 0.8. Amylin (250 nmol l\u20101) also significantly (P &lt; 0.05) reduced supernatant (tartrate\u2010resistant) acid phosphatase in the bone\u2010osteoclast cultures. These measures independently indicate an effect of amylin on osteoclast motility through mechanisms distinct from those of calcitonin, possibly through different selectivities for receptor subtypes, the cyclic AMP\u2010linked 'amylin subtype' and the [Ca2+]i\u2010linked 'calcitonin subtype'.<\/jats:p>","DOI":"10.1113\/expphysiol.1993.sp003679","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T13:26:37Z","timestamp":1418736397000},"page":"183-196","source":"Crossref","is-referenced-by-count":81,"title":["Amylin inhibits bone resorption by a direct effect on the motility of rat osteoclasts"],"prefix":"10.1113","volume":"78","author":[{"given":"AS","family":"Alam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BS","family":"Moonga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PJ","family":"Bevis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CL","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Zaidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1993,3]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1993.sp003679","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1993.sp003679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T19:24:49Z","timestamp":1698175489000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1993.sp003679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,3]]}},"alternative-id":["10.1113\/expphysiol.1993.sp003679"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1993.sp003679","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,3]]}}}