{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T16:20:21Z","timestamp":1698510021521},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1994,1,1]],"date-time":"1994-01-01T00:00:00Z","timestamp":757382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:p>To clarify the influence of blood flow with high\u2010oxygen (O2)\u2010affinity blood on oxygen consumption (VO2) in submaximally exercising skeletal muscle, we perfused the isolated dog gracilis (n = 8) contracting under 1 Hz stimulation alternatively with normal and high\u2010O2\u2010affinity blood, with a constant arterial O2 content (Ca,O2) and varying perfusion rates. The average P50 (oxygen partial pressure (PO2) for half\u2010saturation at pH 7.40, PCO2 of 40 mmHg at 37 degrees C) of the high\u2010O2\u2010affinity blood prepared by carbamylation was 15.5 mmHg, and that of the normal blood 33.7 mmHg. With normal blood perfusion, the average VO2 above 6 ml min\u20101 (100 g)\u20101 of O2 delivery (Ca,O2 x flow) was 4.38 ml min\u20101 (100 g)\u20101 (range 4.17\u20134.68 ml min\u20101 (100 g)\u20101, and VO2 at the O2 delivery range of 6\u20135 and 4\u20102.5 ml min\u20101 (100 g)\u20101 decreased to 3.96 and 2.43 ml min\u20101 (100 g)\u20101, respectively. The PO2 of venous effluent (Pv,O2) at the O2 delivery of 6 ml min\u20101 (100 g)\u20101 was 33 mmHg. With low\u2010P50 blood perfusion, VO2 was significantly less than with normal blood, both below the O2 delivery level of 6 ml min\u20101 (100 g)\u20101 and above it, even in the fairly high O2 delivery range of 8.5\u201312 ml min\u20101 (100 g)\u20101 (P &lt; 0.05). Thus, high blood flow did not compensate for the reduced VO2 caused by high\u2010O2\u2010affinity blood. At values of Pv,O2 less than 33 mmHg, VO2 with low\u2010P50 blood was not significantly different from that with normal blood (P &gt; 0.05). The reduced VO2 in submaximally exercising skeletal muscle might be due to a slower O2 dissociation from the high\u2010O2\u2010affinity red cells and to a limited O2 diffusion resulting from the lower Pv,O2 value (which reflects mean end\u2010capillary PO2).<\/jats:p>","DOI":"10.1113\/expphysiol.1994.sp003743","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T12:59:30Z","timestamp":1418734770000},"page":"71-80","source":"Crossref","is-referenced-by-count":3,"title":["High affinity of blood for oxygen reduces oxygen uptake in contracting canine gracilis muscle"],"prefix":"10.1113","volume":"79","author":[{"given":"H","family":"Kohzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Enoki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Sakata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Ohga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,1]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1994.sp003743","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1994.sp003743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T14:46:32Z","timestamp":1698158792000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1994.sp003743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1113\/expphysiol.1994.sp003743"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1994.sp003743","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}