{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T11:54:36Z","timestamp":1699962876146},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1994,5,1]],"date-time":"1994-05-01T00:00:00Z","timestamp":767750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:p>The effect of infusion (2 h) of various sugars (20%, w\/v) or early diabetes mellitus (2 h following injection of streptozotocin) on the potential difference (PD) across the brush border of rat jejunum has been studied in vitro. Infusion of glucose or galactose resulted in hyperpolarization of the brush\u2010border PD (\u201055.2 and \u201054.9 mV respectively) compared with mannitol\u2010infused animals (\u201047.7 mV). Infusion of fructose and alpha\u2010methyl glucoside was without effect on PD. Ion substitution experiments showed that the glucose infusion\u2010induced hyperpolarization was due either to a decrease in Cl\u2010 conductance and\/or increased K+ conductance of the brush\u2010border membrane. Na+ conductance appeared to be unaltered in these experiments. Since galactose infusion hyperpolarized PD without elevating plasma glucose or insulin levels, it seems that neither glucose nor insulin alone is responsible for the significantly greater PD. Treatment with streptozotocin produced glycosuria and hyperglycaemia within approximately 90 min and animals were used 30\u201060 min later. PD in streptozotocin\u2010treated animals (\u201051.8 mV) was significantly greater than that measured in diluent\u2010injected animals (\u201045.3 mV, P &lt; 0.001). However, unlike the situation with sugar infusion, ion substitution experiments showed that the hyperpolarization seen in diabetic animals was due to a decreased Na+ conductance of the brush\u2010border membrane. Since the magnitude of the brush\u2010border PD dictates the electrochemical driving force for Na(+)\u2010sugar cotransport, our data represent the first evidence for an effect of acute hyperglycaemia on the capacity for brush border sugar uptake. However, the enterocyte and systemic factors responsible for the membrane hyperpolarization are unclear.<\/jats:p>","DOI":"10.1113\/expphysiol.1994.sp003776","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T13:08:12Z","timestamp":1418735292000},"page":"415-421","source":"Crossref","is-referenced-by-count":4,"title":["The effect of rapid changes in plasma sugar concentration on the brush\u2010border potential difference in rat jejunum"],"prefix":"10.1113","volume":"79","author":[{"given":"PA","family":"Sharp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ES","family":"Debnam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,5]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1994.sp003776","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1994.sp003776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T03:20:04Z","timestamp":1698204004000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1994.sp003776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,5]]}},"alternative-id":["10.1113\/expphysiol.1994.sp003776"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1994.sp003776","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5]]}}}