{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:37:31Z","timestamp":1761709051842},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1996,5,1]],"date-time":"1996-05-01T00:00:00Z","timestamp":830908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1996,5]]},"abstract":"<jats:p>This study investigates the mechanism of magnesium (Mg2+) transport (efflux) from the exocrine rat pancreas. Permeabilized pancreatic acini were loaded with Mg2+ by employing a high\u2010Mg2+ (12 mM) buffer containing A23187 (6 microM). Net Mg2+ efflux was measured using the technique of atomic absorbance spectrophotometry. Incubation of preloaded acini in a buffer deficient in Mg2+ resulted in a large and time\u2010dependent release of Mg2+ with maximal efflux occurring within 40 min. Pretreatment of loaded acini with bumetanide, SITS or ouabain had no significant effect on Mg2+ efflux. In contrast, when acini were pretreated with 10 mM dinitrophenol, 10(\u20104) M amiloride, 1 mM lidocaine or 1 mM quinidine there were significant (P &lt; 0.001) decreases in net Mg2+ efflux. Replacement of extracellular sodium [Na+]o with either N\u2010methyl\u2010D\u2010glucamine (NMDG), Tris or choline resulted in a significant (P &lt; 0.001) inhibition of Mg2+ efflux. The results of this study indicate that Mg2+ transport (efflux) in pancreatic acinar cells may not be associated with the Na(+)\u2010K(+)\u2010ATPase, the Na(+)\u2010K(+)\u2010Cl\u2010 cotransporter or the anion exchanger, but with a Na(2+)\u2010sensitive Mg2+ transport system.<\/jats:p>","DOI":"10.1113\/expphysiol.1996.sp003941","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T12:25:28Z","timestamp":1418732728000},"page":"367-374","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of a sodium\u2010dependent magnesium efflux from magnesium\u2010loaded rat pancreatic acinar cells"],"prefix":"10.1113","volume":"81","author":[{"given":"DM","family":"Wisdom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MM","family":"Geada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,5]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1996.sp003941","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1996.sp003941","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T06:47:51Z","timestamp":1698389271000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1996.sp003941"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,5]]}},"alternative-id":["10.1113\/expphysiol.1996.sp003941"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1996.sp003941","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5]]}}}