{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:58:07Z","timestamp":1762113487029},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1996,7,1]],"date-time":"1996-07-01T00:00:00Z","timestamp":836179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1996,7]]},"abstract":"<jats:p>The role of bicarbonate as a hydrogen ion buffer has been investigated using the fluorescent dye BCECF in individual rat cerebellar, hippocampal and neocortical neurones maintained in culture. The steady\u2010state intracellular pH (pHi) was estimated to be 7.07 +\/\u2212 0.05 (n = 22) in CO2\u2010HCO3(\u2010)\u2010buffered media. Buffering power (beta) estimated from the addition and removal of weak bases was ca 10 mM (pH unit)\u20101 and was found to be similar in both CO2\u2010HCO3(\u2010)\u2010 and Hepes\u2010buffered media. The membrane\u2010permeant carbonic anhydrase inhibitor, acetazolamide (10\u201320 microM), did not affect estimates of beta. The results indicate that CO2\u2010HCO3\u2010 does not act as an open buffer system in these neurones.<\/jats:p>","DOI":"10.1113\/expphysiol.1996.sp003963","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T12:31:01Z","timestamp":1418733061000},"page":"623-632","source":"Crossref","is-referenced-by-count":11,"title":["On the role of bicarbonate as a hydrogen ion buffer in rat CNS neurones"],"prefix":"10.1113","volume":"81","author":[{"given":"BJ","family":"Amos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Pocock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CD","family":"Richards","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,7]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1996.sp003963","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1996.sp003963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T17:04:22Z","timestamp":1698339862000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1996.sp003963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,7]]}},"alternative-id":["10.1113\/expphysiol.1996.sp003963"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1996.sp003963","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7]]}}}