{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T15:54:31Z","timestamp":1720713271627},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1996,7,1]],"date-time":"1996-07-01T00:00:00Z","timestamp":836179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1996,7]]},"abstract":"<jats:p>The secretory effects of 5\u2010hydroxydopamine (5\u2010OHDA) were tested in Nembutal\u2010anaesthetized adult male Sprague\u2010Dawley rats injected I.V. over a wide range of doses, with and without various autonomic antagonists and Ca2+ channel blockers. Polyacrylamide disc gel (15%) and iso\u2010electric focusing (IEF) electrophoresis by the PhastSystem were used to separate and determine the types of protein in submandibular saliva. Amylase activity of parotid saliva was determined by the blue dextran method. Salivation by the submandibular glands following application of 5\u2010OHDA was completely abolished by both prazosin and propranolol, whereas salivation by the parotid glands was completely abolished by propranolol alone. Following application of 5\u2010OHDA, there was a dose\u2010related increase in flow rates and total output of protein, but not in the protein concentration and amylase activity, from both salivary glands. The effect of 5\u2010OHDA on submandibular saliva was significantly reduced by alpha\u2010adrenoceptor blockers, but not by beta\u2010adrenoceptor and cholinergic blockers, nor by any Ca2+ channel blocker. The effect of 5\u2010OHDA on the parotid gland was not significantly altered by atropine and phentolamine. However, after pretreatment with reserpine, a 95% reduction was observed in the salivation from the submandibular gland. This implies that 5\u2010OHDA is mostly acting indirectly via release of noradrenaline. The proteins in submandibular saliva following treatment with 5\u2010OHDA alone or 5\u2010OHDA in combination with beta\u2010adrenoceptor blockers were mainly of the alpha\u2010type, whereas after treatment with 5\u2010OHDA in combination with alpha\u2010adrenoceptor blockers they were of the beta\u2010type. The alpha\u2010type was found in saliva after treatment with each of three Ca2+ channel blockers.<\/jats:p>","DOI":"10.1113\/expphysiol.1996.sp003965","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T12:31:01Z","timestamp":1418733061000},"page":"645-653","source":"Crossref","is-referenced-by-count":2,"title":["The effects of 5\u2010hydroxydopamine on salivary flow rates and protein secretion by the submandibular and parotid glands of rats"],"prefix":"10.1113","volume":"81","author":[{"given":"K","family":"Abe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Tashiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Okina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Nose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SF","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,7]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1996.sp003965","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1996.sp003965","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T17:04:25Z","timestamp":1698339865000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1996.sp003965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1996,7]]}},"alternative-id":["10.1113\/expphysiol.1996.sp003965"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1996.sp003965","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7]]}}}