{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T09:13:30Z","timestamp":1698484410145},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1997,1,1]],"date-time":"1997-01-01T00:00:00Z","timestamp":852076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>In this study, K+ concentration was measured in effluent samples from superfused guinea\u2010pig pancreatic pieces in control conditions and during stimulation with ACh, employing the technique of flame photometry. ACh (10(\u20107)\u201010(\u20105) M) evoked a dose\u2010dependent and sustained increase in K+ concentration in the effluent (K+ release). The removal of Ca2+ from the superfusing medium and the addition of 10(\u20104) M EGTA caused a significant (P &lt; 0.05) reduction in the ACh\u2010evoked K+ efflux. Replacement of extracellular Cl\u2010 in the superfusing physiological salt solution with NO3\u2010 abolished the ACh\u2010induced K+ efflux. In contrast, when Cl\u2010 was replaced with Br\u2010, ACh still evoked marked K+ release. Pretreatment of pancreatic segments with the loop diuretic furosemide (10(\u20104) M) resulted in an inhibition of K+ efflux elicited by ACh. Stimulation of pancreatic segments with the Na(+)\u2010K(+)\u2010ATPase inhibitor ouabain (10(\u20103) M) caused a large efflux of K+. In the continuous presence of ouabain, ACh application elicited no further change in the K+ release. The results indicate that ACh\u2010evoked K+ release from guinea\u2010pig pancreatic segments is sensitive to ouabain, Cl\u2010, furosemide and extracellular Ca2+ and that only the basal efflux is augmented by ouabain. The findings provide further evidence that a diuretic\u2010sensitive coupled Na(+)\u2010K(+)\u2010Cl\u2010 cotransport system operates in the guinea\u2010pig pancreas, as it does in other similar transporting epithelia, to bring about K+ mobilization.<\/jats:p>","DOI":"10.1113\/expphysiol.1997.sp004004","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T11:59:24Z","timestamp":1418731164000},"page":"149-159","source":"Crossref","is-referenced-by-count":1,"title":["Acetylcholine\u2010evoked potassium transport in the isolated guinea\u2010pig pancreas"],"prefix":"10.1113","volume":"82","author":[{"given":"JA","family":"Rosado","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GM","family":"Salido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LJ","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,1]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1997.sp004004","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1997.sp004004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T02:00:10Z","timestamp":1698458410000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1997.sp004004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1]]}},"alternative-id":["10.1113\/expphysiol.1997.sp004004"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1997.sp004004","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1]]}}}