{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:40:35Z","timestamp":1762101635500},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1997,1,1]],"date-time":"1997-01-01T00:00:00Z","timestamp":852076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>Osmotic stress was applied to the perfused rat submandibular gland during steady\u2010state fluid secretion. Alterations of perfusate osmolarity, by addition or withdrawal of sucrose or NaCl, caused transient changes in secretory rate during continuous stimulation with 1 microM acetylcholine (ACh). Hyposmotic perfusates transiently increased, and hyperosmotic perfusates transiently reduced, the secretory rate. The transients were attributed to changes in osmotic flow resulting from changes in the instantaneous transepithelial osmotic gradient. The time course of the change in interstitial osmolarity was determined by using a Cl\u2010 electrode to record the changes in interstitial Cl\u2010 concentration following a step change in perfusate Cl\u2010 concentration. From the calculated changes in interstitial osmolarity and the resulting changes in secretory rate, the osmotic water permeability of the secretory pathway was estimated to be greater than 15.0 +\/\u2212 1.2 microliter (mosmol 1\u20101)\u20101 min\u20101 (g wet weight)\u20101 (9.8 x 10(\u20106) +\/\u2212 0.8 x 10(\u20106) l atm\u20101s\u20101g\u20101). The transepithelial gradient required to sustain steady state, ACh\u2010evoked secretion would therefore be less than 16 mosmol l\u20101 NaCl, which is consistent with previous micropuncture data indicating that the luminal fluid is approximately isosmotic.<\/jats:p>","DOI":"10.1113\/expphysiol.1997.sp004015","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T11:59:24Z","timestamp":1418731164000},"page":"55-70","source":"Crossref","is-referenced-by-count":8,"title":["Osmotic flow transients during acetylcholine stimulation in the perfused rat submandibular gland"],"prefix":"10.1113","volume":"82","author":[{"given":"T","family":"Nakahari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MC","family":"Steward","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Imai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,1]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1997.sp004015","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1997.sp004015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T02:00:15Z","timestamp":1698458415000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1997.sp004015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1]]}},"alternative-id":["10.1113\/expphysiol.1997.sp004015"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1997.sp004015","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1]]}}}