{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:44:05Z","timestamp":1758397445089},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1997,5,1]],"date-time":"1997-05-01T00:00:00Z","timestamp":862444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1997,5]]},"abstract":"<jats:p>The effects of the ATP\u2010sensitive K+ channel (KATP channel) opener cromakalim on excitation\u2010contraction (E\u2010C) coupling were studied in skeletal muscle during fatiguing and non\u2010fatiguing activity. Intracellular calcium concentration ([Ca2+]i) was monitored using the fluorescent indicator fura\u20102 in isolated single skeletal muscle fibres enzymatically dissociated from rat flexor digitorum brevis. A protocol of tetanic stimulation (50 Hz for 300 ms) with progressively shorter durations between tetani was used to induce E\u2010C coupling failure in these cells. Cromakalim (100\u2010800 microM) had little effect on peak [Ca2+]i during twitch and non\u2010fatiguing tetanic stimulation. However, with 0.4 s between tetani, 100 microM cromakalim decreased peak tetanic [Ca2+]i from 1.47 +\/\u08a4 0.11 microM to 8.35 +\/\u08a4 55 nM, but did not affect resting [Ca2+]i (control, 220 +\/\u08a4 40 nM; with cromakalim, 171 +\/\u08a4 33 nM). Cyanide (2 mM) decreased tetanic [Ca2+]i and increased resting [Ca2+]i during the stimulus protocol; with 0.4 s between tetani, peak [Ca2+]i was 820 +\/\u08a4 50 nM and resting [Ca2+]i was 443 +\/\u08a4 32 nM. The ability of cromakalim to inhibit E\u2010C coupling was enhanced by the presence of cyanide. Complete blockade of metabolism by cyanide and iodoactetate (0.1 mM) caused a marked rise in resting [Ca2+]i and inhibition of the tetanic rise of [Ca2+]i. With cromakalim (100 microM) present, E\u2010C coupling failed during metabolic blockade but without a significant increase in resting [Ca2+]i. These results are consistent with a role for the KATP channel in the failure of Ca2+ release during fatigue.<\/jats:p>","DOI":"10.1113\/expphysiol.1997.sp004040","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T12:06:00Z","timestamp":1418731560000},"page":"469-483","source":"Crossref","is-referenced-by-count":11,"title":["The effect of cromakalim on intracellular [Ca2+] in isolated rat skeletal muscle during fatigue and metabolic blockade"],"prefix":"10.1113","volume":"82","author":[{"given":"FL","family":"Burton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GL","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,5]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1997.sp004040","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1997.sp004040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T13:17:00Z","timestamp":1698412620000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1997.sp004040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,5]]}},"alternative-id":["10.1113\/expphysiol.1997.sp004040"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1997.sp004040","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,5]]}}}