{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T09:13:19Z","timestamp":1698484399505},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1997,9,1]],"date-time":"1997-09-01T00:00:00Z","timestamp":873072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>A systematic examination has been made of the active and passive electrophysiological properties and synaptic inputs of forty\u2010four randomly impaled submucosal neurones in the proximal colon of the guinea\u2010pig to compare these characteristics directly with those of submucosal neurones in the caecum (n = 70) and descending colon (n = 45). Within each of the three electrophysiological classes of submucosal neurones identified (S, S\/AH and AH), no statistically significant regional differences were found with respect to the resting membrane potential, membrane time constant or input resistance between neurones of the proximal colon, descending colon and caecum. Of submucosal neurones from the proximal colon, forty\u2010three of forty\u2010four (98%) received fast excitatory synaptic potentials (fast EPSPs); thirty\u2010nine (91%) were S neurones and the others were S\/AH neurones; only one of the forty\u2010four cells (2%) was an AH neurone. An idazoxan\u2010sensitive slow inhibitory postsynaptic potential (slow IPSP) was induced in thirty of forty\u2010three S and S\/AH neurones (70%) of the proximal colon, compared with sixty\u2010one of sixty\u2010six caecal neurones (92%) and twelve of forty\u2010one neurones (29%) in the descending colon. The mean (+\/\u2010 S.E.M.) amplitude of the slow IPSP in proximal colonic neurones was 17 +\/\u08a4 1 mV (range, 6\u201030 mV; n = 30), compared with the significantly larger synaptic response (25 +\/\u08a4 1 mV; range, 7\u201038 mV; n = 66; P &lt; 0.05) recorded in the caecum; the mean slow IPSP amplitude in the descending colon was significantly smaller (12 +\/\u08a4 2 mV; range, 5\u201027 mV; n = 12; P &lt; 0.05) than that in the caecum. In the proximal colon and caecum, only those neurones with a slow IPSP had a hyperpolarizing response to noradrenaline, whereas about 50% of those neurons of the descending colon that lacked a slow IPSP were hyperpolarized by noradrenaline, acting via alpha 2\u2010adrenoceptors. Thus, the electrophysiological characteristics of the submucosal neurones of the proximal colon more closely resemble those of the caecum than those of the descending colon, of which many do not have a functional noradrenergic synaptic input. Furthermore, the results confirm that there are fundamental regional differences in the guinea\u2010pig large intestine with respect to the synaptic organization of submucosal neurones of particular electrophysiological classes.<\/jats:p>","DOI":"10.1113\/expphysiol.1997.sp004069","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T12:12:16Z","timestamp":1418731936000},"page":"859-870","source":"Crossref","is-referenced-by-count":4,"title":["Electrophysiological characteristics of submucosal neurones in the proximal colon of guinea\u2010pigs: comparisons with caecum and descending colon"],"prefix":"10.1113","volume":"82","author":[{"given":"SM","family":"Cunningham","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Hirai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Mihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GM","family":"Lees","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,9]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1997.sp004069","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1997.sp004069","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T12:32:39Z","timestamp":1698409959000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1997.sp004069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1113\/expphysiol.1997.sp004069"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1997.sp004069","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}