{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T12:49:49Z","timestamp":1773406189728,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:p>Short chain fatty acids (SCFAs) and CO2 have been shown to stimulate net Mg2+ efflux from the isolated reticulorumen in vivo. To investigate the underlying mechanisms of Mg2+ transport we performed Ussing chamber and microelectrode experiments and measured 28Mg2+ fluxes across sheep rumen epithelium in vitro. In the presence of SCFAs mucosal\u2010to\u2010serosal Mg2+ flux (Jm\u2010sMg) amounted to 82.3 +\/\u2212 7.8 nmol cm\u20102 h\u20101 and serosal\u2010to\u2010mucosal Mg2+ flux (Js\u2010mMg) to 3.2 +\/\u2212 0.7 nmol cm\u20102 h\u20101. Replacing SCFAs with gluconate caused a 50% reduction of Jm\u2010sMg, whereas Js\u2010mMg was not affected. Among the SCFAs, n\u2010butyrate was more effective in stimulating Jm\u2010sMg than acetate, propionate or iso\u2010butyrate. Eliminating HCO3(\u2010)\u2010CO2 from SCFA\u2010containing solutions did not affect Mg2+ fluxes, whereas the same replacement in SCFA\u2010free solutions led to a further reduction in Jm\u2010sMg. Jm\u2010sMg decreased after the addition of ethoxyzolamide to SCFA\u2010free, bicarbonate buffered solutions. Decreasing mucosal pH from 6.4 to 5.4 increased Jm\u2010sMg in SCFA\u2010free, bicarbonate buffered solutions. SCFAs had no effect on the apical membrane potential of rumen epithelial cells. The experiments show that both SCFAs and CO2 stimulate Mg2+ transport through an increase in Jm\u2010sMg, most probably via stimulation of a Mg(2+)\u20102H+ exchange mechanism. SCFAs may have additional metabolic effects on Mg2+ transport.<\/jats:p>","DOI":"10.1113\/expphysiol.1998.sp004098","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T11:42:50Z","timestamp":1418730170000},"page":"155-164","source":"Crossref","is-referenced-by-count":35,"title":["Effects of short chain fatty acids and carbon dioxide on magnesium transport across sheep rumen epithelium"],"prefix":"10.1113","volume":"83","author":[{"given":"S","family":"Leonhard-Marek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Gabel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Martens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,3]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1998.sp004098","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1998.sp004098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T01:26:30Z","timestamp":1698456390000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1998.sp004098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,3]]}},"alternative-id":["10.1113\/expphysiol.1998.sp004098"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1998.sp004098","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3]]}}}