{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:17:26Z","timestamp":1761059846514},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:p>Gut endocrine cells possess the capacity to take up and decarboxylate biogenic amine precursors. Decarboxylation is mediated by aromatic L\u2010amino acid decarboxylase (AADC) which is encoded by mRNAs differing in their 5' untranslated regions (UTR) depending on the usage of alternative first exons, 1a and 1b, each with its own acceptor site (\u221213 and \u22128 bases relative to the translation start site, respectively). We describe here a novel splice variant of exon 1a\u2010AADC mRNA in rat antral mucosa. Both exon 1a and 1b mRNAs were expressed in rat antral mucosa, but the 1a form was spliced into the acceptor site usually associated with exon 1b (\u22128). An enteroendocrine cell line (STC\u20101) expressed exon 1a or exon 1b mRNAs spliced into the \u20108 acceptor site of exon 2. Transient transfection of a range of cell lines with reporter constructs revealed that all three 5' UTRs efficiently supported expression of the Luciferase reporter. There is therefore a novel, functional 57' UTR of AADC mRNA in rat antral mucosa; alternative AADC splice variants could provide the capacity for control at the level of mRNA translation.<\/jats:p>","DOI":"10.1113\/expphysiol.1998.sp004143","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T11:51:28Z","timestamp":1418730688000},"page":"617-627","source":"Crossref","is-referenced-by-count":7,"title":["Novel form of L\u2010aromatic amino acid decarboxylase mRNA in rat antral mucosa"],"prefix":"10.1113","volume":"83","author":[{"given":"PK","family":"Djali","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Dimaline","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GJ","family":"Dockray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,9]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1998.sp004143","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1998.sp004143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T01:47:10Z","timestamp":1698457630000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1998.sp004143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["10.1113\/expphysiol.1998.sp004143"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1998.sp004143","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]}}}