{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T15:13:51Z","timestamp":1698506031092},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1998,9,1]],"date-time":"1998-09-01T00:00:00Z","timestamp":904608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:p>Potassium transport was measured in isolated chicken colonocytes using 85Rb+ as a tracer for K+. Rb+ was determined by atomic absorption spectrometry. The results revealed that net K+ uptake occurred via at least four mechanisms: (i) Na+,K(+)\u2010ATPase, (ii) K(+)\u2010ATPase, (iii) Na(+)\u2010K(+)\u20102Cl\u2010 cotransport system and (iv) a mechanism(s) which is resistant to both ouabain and bumetanide. The rate of K+(Rb+) efflux is stimulated by the calcium ionophore A23187, inhibited by either quinine, verapamil or Ba2+, and unaffected by either apamin, 3,4\u2010diaminopyridine (3,4\u2010DAP), H2\u2010DIDS or bumetanide. The A23187\u2010induced increase in K+(Rb+) efflux was abolished by apamin. These findings suggest that K+(Rb+) efflux from chicken colonocytes occurs at least in part through Ca(2+)\u2010activated K+ channels. The present results also show that all these K+ transport systems are involved in cell volume regulation. Thus, external hyposmolarity decreased net K+(Rb+) uptake mediated by Na+,K(+)\u2010ATPase, K(+)\u2010ATPase and the Na(+)\u2010K(+)\u20102Cl\u2010 cotransporter and increased K+(Rb+) efflux rate. The opposite was observed under hyperosmotic conditions.<\/jats:p>","DOI":"10.1113\/expphysiol.1998.sp004144","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T11:51:28Z","timestamp":1418730688000},"page":"629-638","source":"Crossref","is-referenced-by-count":3,"title":["K+ transport in colonocytes isolated from the chick: effect of anisosmotic buffers"],"prefix":"10.1113","volume":"83","author":[{"given":"ML","family":"Calonge","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Cano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AA","family":"Ilundain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,9]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1998.sp004144","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1998.sp004144","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T01:47:05Z","timestamp":1698457625000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1998.sp004144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["10.1113\/expphysiol.1998.sp004144"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1998.sp004144","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]}}}