{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T23:27:49Z","timestamp":1774049269369,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:p><jats:bold>In anaesthetized female rats, the \u03b2\u2010adrenoceptor agonist isoprenaline was intravenously infused (20 \u03bcg kg<jats:sup>\u22121<\/jats:sup> min<jats:sup>\u22121<\/jats:sup>) for 30 min or the ascending cervical sympathetic nerve trunk was intermittently stimulated (50 Hz, 1 s every tenth second) on one side for 30 min. The incorporation of [<jats:sup>3<\/jats:sup>H]leucine into trichloroacetic acid (TCA)\u2010insoluble material was used as an index of protein synthesis. In response to isoprenaline, the [<jats:sup>3<\/jats:sup>H]leucine incorporation increased by 79% in the parotid glands and by 82% in the submandibular glands. The neuronal type NO\u2010synthase inhibitor N\u2010PLA, reduced (<jats:italic>P<\/jats:italic> &lt; 0.001) this response to 26% and 20%, respectively. Sympathetic stimulation under \u03b1\u2010adrenoceptor blockade increased the [<jats:sup>3<\/jats:sup>H]leucine incorporation by 192% in the parotid glands and by 35% in the submandibular glands. N\u2010PLA reduced the corresponding percentage figures to 86% (<jats:italic>P<\/jats:italic> &lt; 0.01) and 8% (<jats:italic>P<\/jats:italic> &lt; 0.05). When tested in the parotid glands, the non\u2010selective NO\u2010synthase inhibitor <jats:sc>L<\/jats:sc>\u2010NAME reduced (<jats:italic>P<\/jats:italic> &lt; 0.01) the nerve\u2010evoked response to 91%. The increase in [<jats:sup>3<\/jats:sup>H]leucine incorporation in response to sympathetic stimulation under \u03b2\u2010adrenoceptor blockade was not affected by N\u2010PLA in the parotid (139%<jats:italic>versus<\/jats:italic> 144%) and submandibular glands (39%<jats:italic>versus<\/jats:italic> 34%). In non\u2010stimulated glands, the [<jats:sup>3<\/jats:sup>H]leucine incorporation was not influenced by the NO\u2010synthase inhibitors. In conclusion, \u03b2\u2010adrenoceptor mediated salivary gland protein synthesis is largely dependent on NO generation by neuronal type NO\u2010synthase, most likely of parenchymal origin.<\/jats:bold><\/jats:p>","DOI":"10.1113\/expphysiol.2003.002648","type":"journal-article","created":{"date-parts":[[2004,2,26]],"date-time":"2004-02-26T18:08:00Z","timestamp":1077818880000},"page":"219-227","source":"Crossref","is-referenced-by-count":12,"title":["Nitric oxide\u2010dependent protein synthesis in parotid and submandibular glands of anaesthetized rats upon sympathetic stimulation or isoprenaline 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