{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T11:28:34Z","timestamp":1697282914049},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,20]],"date-time":"2005-12-20T00:00:00Z","timestamp":1135036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>Ca<jats:sup>2<\/jats:sup><jats:sup>+<\/jats:sup>\u2010regulated exocytosis is enhanced by an autocrine mechanism via the PGE<jats:sub>2<\/jats:sub>\u2013cAMP pathway in antral mucous cells of guinea\u2010pigs. The inhibition of the PGE<jats:sub>2<\/jats:sub>\u2013cAMP pathway by H\u201089 (an inhibitor of protein kinase A, PKA) or aspirin (ASA, an inhibitor of cyclo\u2010oxygenase, COX) decreased the frequency of ACh\u2010stimulated exocytotic events by 60%. Indomethacin (IDM, an inhibitor of COX), however, decreased the frequency of ACh\u2010stimulated exocytotic events only by 30%. Moreover, IDM increased the frequency of ACh\u2010stimulated exocytotic events by 50% in H\u201089\u2010treated or ASA\u2010treated cells. IDM inhibits the synthesis of Prostaglandin (PGG\/H) and (15R)\u201015\u2010hydroxy\u20105,8,11 cis\u201013\u2010trans\u2010eicosatetraenoic acid (15R\u2010HPETE), while ASA inhibits only the synthesis of PGG\/H. Thus, IDM may accumulate arachidonic acid (AA). AACOCF<jats:sub>3<\/jats:sub> or <jats:italic>N<\/jats:italic>\u2010(<jats:italic>p<\/jats:italic>\u2010amylcinnamoyl) anthranilic acid (ACA; both inhibitors of phospholipase A<jats:sub>2<\/jats:sub>, PLA<jats:sub>2<\/jats:sub>), which inhibits AA synthesis, decreased the frequency of ACh\u2010stimulated exocytotic events by 60%. IDM, however, did not increase the frequency in AACOCF<jats:sub>3<\/jats:sub>\u2010treated cells. AA increased the frequency of ACh\u2010stimulated exocytotic events in AACOCF<jats:sub>3<\/jats:sub>\u2010 or ASA\u2010treated cells, similar to IDM in ASA\u2010 and H\u201089\u2010treated cells. Moreover, in the presence of AA, IDM did not increase the frequency of ACh\u2010stimulated exocytotic events in ASA\u2010treated cells. The PGE<jats:sub>2<\/jats:sub> release from antral mucosa indicates that inhibition of PLA<jats:sub>2<\/jats:sub> by ACA inhibits the AA accumulation in unstimulated and ACh\u2010stimulated antral mucosa. The dose\u2013response study of AA and IDM demonstrated that the concentration of intracellular AA accumulated by IDM is less than 100 n<jats:sc>m<\/jats:sc>. In conclusion, IDM modulates the ACh\u2010stimulated exocytosis via AA accumulation in antral mucous cells.<\/jats:p>","DOI":"10.1113\/expphysiol.2005.032482","type":"journal-article","created":{"date-parts":[[2005,11,2]],"date-time":"2005-11-02T01:24:42Z","timestamp":1130894682000},"page":"249-259","source":"Crossref","is-referenced-by-count":13,"title":["Enhancement of Ca<sup>2+<\/sup>\u2010regulated exocytosis by indomethacin in guinea\u2010pig antral mucous cells: arachidonic acid 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