{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:20:26Z","timestamp":1789978826105,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p>\n                    In the absence of information on functional manifestations of carotid body (CB) inflammation, we studied an experimental model in which lipopolysaccharide (LPS) administration to pentobarbitone\u2010anaesthetized cats was performed by topical application upon the CB surface or by intravenous infusion (endotoxaemia). The latter caused: (i) disorganization of CB glomoids, increased connective tissue, and rapid recruitment of polymorphonuclear cells into the vascular bed and parenchyma within 4 h; (ii) increased respiratory frequency and diminished ventilatory chemoreflex responses to brief hypoxia (breathing 100% N\n                    <jats:sub>2<\/jats:sub>\n                    for 10 s) and diminished ventilatory chemosensory drive (assessed by 100% O\n                    <jats:sub>2<\/jats:sub>\n                    tests) during normoxia and hypoxia; (iii) tachycardia, increased haematocrit and systemic hypotension in response to LPS\n                    <jats:sc>i.v.<\/jats:sc>\n                    ; and (iv) increased basal frequency of carotid chemosensory discharges during normoxia, but no change in maximal chemoreceptor responses to brief hypoxic exposures. Lipopolysaccharide\u2010induced tachypnoea was prevented by prior bilateral carotid neurotomy. Apoptosis was not observed in CBs from cats subjected to endotoxaemia. Searching for pro\u2010inflammatory mediators, tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) was localized by immunohistochemistry in glomus and endothelial cells; reverse transcriptase\u2010polymerase chain reaction revealed that the CB expresses the mRNAs for both type\u20101 (TNF\u2010R1) and type\u20102 TNF\u2010\u03b1 receptors (TNF\u2010R2); Western blot confirmed a band of the size expected for TNF\u2010R1; and histochemistry showed the presence of TNF\u2010R1 in glomus cells and of TNF\u2010R2 in endothelial cells. Experiments\n                    <jats:italic>in vitro<\/jats:italic>\n                    showed that the frequency of carotid nerve discharges recorded from CBs perfused and superfused under normoxic conditions was not significantly modified by TNF\u2010\u03b1, but that the enhanced frequency of chemosensory discharges recorded along responses to hypoxic stimulation was transiently diminished in a dose\u2010dependent manner by TNF\u2010\u03b1 injections. The results suggest that the CB may operate as a sensor for immune signals, that the CB exhibits histological features of acute inflammation induced by LPS, that TNF\u2010\u03b1 may participate in LPS\u2010induced changes in chemosensory activity and that some pathophysiological reactions to high levels of LPS in the bloodstream may originate from changes in CB function.\n                  <\/jats:p>","DOI":"10.1113\/expphysiol.2008.041152","type":"journal-article","created":{"date-parts":[[2008,6,18]],"date-time":"2008-06-18T10:02:49Z","timestamp":1213783369000},"page":"892-907","source":"Crossref","is-referenced-by-count":69,"title":["Lipopolysaccharide\u2010induced carotid body inflammation in cats: functional manifestations, histopathology and involvement of tumour necrosis factor\u2010\u03b1"],"prefix":"10.1113","volume":"93","author":[{"given":"Ricardo","family":"Fern\u00e1ndez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Rey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula P.","family":"Cort\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin 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