{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T02:01:12Z","timestamp":1784685672773,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,7,14]],"date-time":"2008-07-14T00:00:00Z","timestamp":1215993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p>With the advent of numerous candidate drugs for therapy in cystic fibrosis (CF), there is an urgent need for easily interpretable assays for testing their therapeutic value. Defects in the cystic fibrosis transmembrane conductance regulator (CFTR) abolished \u03b2\u2010adrenergic but not cholinergic sweating in CF. Therefore, the \u03b2\u2010adrenergic response of the sweat gland may serve both as an<jats:italic>in vivo<\/jats:italic>diagnostic tool for CF and as a quantitative assay for testing the efficacy of new drugs designed to restore CFTR function in CF. Hence, with the objective of defining optimal conditions for stimulating \u03b2\u2010adrenergic sweating, we have investigated the components and pharmacology of sweat secretion using cell cultures and intact sweat glands. We studied the electrical responses and ionic mechanisms involved in \u03b2\u2010adrenergic and cholinergic sweating. We also tested the efficacy of different \u03b2\u2010adrenergic agonists. Our results indicated that in normal subjects the cholinergic secretory response is mediated by activation of Ca<jats:sup>2+<\/jats:sup>\u2010dependent Cl<jats:sup>\u2212<\/jats:sup>conductance as well as K<jats:sup>+<\/jats:sup>conductances. In contrast, the \u03b2\u2010adrenergic secretory response is mediated exclusively by activation of a cAMP\u2010dependent CFTR Cl<jats:sup>\u2212<\/jats:sup>conductance without a concurrent activation of a K<jats:sup>+<\/jats:sup>conductance. Thus, the electrochemical driving forces generated by \u03b2\u2010adrenergic agonists are significantly smaller compared with those generated by cholinergic agonists, which in turn reflects in smaller \u03b2\u2010adrenergic secretory responses compared with cholinergic secretory responses. Furthermore, the \u03b2\u2010adrenergic agonists, isoproprenaline and salbutamol, induced sweat secretion only when applied in combination with an adenylyl cyclase activator (forskolin) or a phosphodiesterase inhibitor (3\u2010isobutyl\u20101\u2010methylxanthine, aminophylline or theophylline). We surmise that to obtain consistent \u03b2\u2010adrenergic sweat responses, levels of intracellular cAMP above that achievable with a \u03b2\u2010adrenergic agonist alone are essential. \u03b2\u2010Adrenergic secretion can be stimulated<jats:italic>in vivo<\/jats:italic>by concurrent iontophoresis of these drugs in normal, but not in CF, subjects.<\/jats:p>","DOI":"10.1113\/expphysiol.2008.042283","type":"journal-article","created":{"date-parts":[[2008,4,26]],"date-time":"2008-04-26T01:48:51Z","timestamp":1209174531000},"page":"969-981","source":"Crossref","is-referenced-by-count":34,"title":["Iontophoretic \u03b2\u2010adrenergic stimulation of human sweat glands: possible assay for cystic fibrosis transmembrane conductance regulator activity<i>in vivo<\/i>"],"prefix":"10.1113","volume":"93","author":[{"given":"A. K. M.","family":"Shamsuddin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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