{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:50:07Z","timestamp":1760982607507},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,8,14]],"date-time":"2008-08-14T00:00:00Z","timestamp":1218672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p>Intracellular pH (pH<jats:sub>i<\/jats:sub>) was measured in isolated <jats:italic>Xenopus laevis<\/jats:italic> single myofibres at the onset of contractions, with and without glycolytic blockade, to investigate the time course of glycolytic activation. Single myofibres (<jats:italic>n<\/jats:italic>= 8; CON) were incubated in 2\u2032,7\u2032\u2010bis(carboxyethyl)\u20105(6)\u2010carboxyfluorescein acetoyxmethyl ester (10 \u03bc<jats:sc>m<\/jats:sc>; for fluorescence measurement of pH<jats:sub>i<\/jats:sub>) and stimulated for 15 s at 0.67 Hz in anoxia in the absence (control condition; CON) and presence of a glycolytic inhibitor (1 m<jats:sc>m<\/jats:sc> iodoacetic acid; IAA). Intracellular pH<jats:sub>i<\/jats:sub> and tension were continuously recorded, and the differences in pH<jats:sub>i<\/jats:sub> between conditions were used to estimate the activation time of glycolysis. An immediate and steady increase in pH<jats:sub>i<\/jats:sub> (initial alkalosis) at the onset of contractions was similar between CON and IAA trials for the first 9 s of the contractile bout. However, from six contractions (\u223c10 s) throughout the remainder of the bout, IAA demonstrated a continued rise in pH<jats:sub>i<\/jats:sub>, in contrast to a progressive decrease in pH<jats:sub>i<\/jats:sub> in CON (<jats:italic>P<\/jats:italic> &lt; 0.05). These results demonstrate, with high temporal resolution, that glycolysis is activated within six contractions (10 s at 0.67 Hz) in single <jats:italic>Xenopus<\/jats:italic> skeletal muscle fibres.<\/jats:p>","DOI":"10.1113\/expphysiol.2008.042440","type":"journal-article","created":{"date-parts":[[2008,5,31]],"date-time":"2008-05-31T01:09:56Z","timestamp":1212196196000},"page":"1076-1084","source":"Crossref","is-referenced-by-count":7,"title":["Glycolytic activation at the onset of contractions in isolated <i>Xenopus laevis<\/i> single myofibres"],"prefix":"10.1113","volume":"93","author":[{"given":"Brandon","family":"Walsh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Creed M.","family":"Stary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard 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