{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:11:42Z","timestamp":1792005102471,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,11,18]],"date-time":"2008-11-18T00:00:00Z","timestamp":1226966400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>We aimed to quantify the Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> exchange occurring during exercise in rat skeletal muscle <jats:italic>in vivo<\/jats:italic>. Intracellular Na<jats:sup>+<\/jats:sup> and K<jats:sup>+<\/jats:sup> content, Na<jats:sup>+<\/jats:sup> permeability (<jats:sup>22<\/jats:sup>Na<jats:sup>+<\/jats:sup> influx), Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> pump activity (ouabain\u2010sensitive <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake) and Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> pump \u03b1<jats:sub>2<\/jats:sub> subunit content ([<jats:sup>3<\/jats:sup>H]ouabain binding) were measured. Six\u2010week\u2010old rats rested (control animals) or performed intermittent running for 10\u201360 min and were then killed or were killed at 15 or 90 min following 60 min exercise. In the soleus muscle, intracellular Na<jats:sup>+<\/jats:sup> was 80% higher than in control rats after 60 min exercise, was still elevated (38%) after 15 min rest and returned to control levels after 90 min rest. Intracellular K<jats:sup>+<\/jats:sup> showed corresponding decreases after 15\u201360 min exercise, returning to control levels 90 min postexercise. Exercise induced little change in Na<jats:sup>+<\/jats:sup> and K<jats:sup>+<\/jats:sup> in the extensor digitorum longus muscle (EDL). In soleus, the exercise\u2010induced rise in Na<jats:sup>+<\/jats:sup> and reduction in K<jats:sup>+<\/jats:sup> were augmented by pretreatment with ouabain or by reducing the content of muscular Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> pumps by prior K<jats:sup>+<\/jats:sup> depletion of the animals. Fifteen minutes after 60 min exercise, ouabain\u2010sensitive <jats:sup>86<\/jats:sup>Rb<jats:sup>+<\/jats:sup> uptake in the soleus was increased by 30% but was unchanged in EDL, and there was no effect of exercise on [<jats:sup>3<\/jats:sup>H]ouabain binding measured <jats:italic>in vitro<\/jats:italic> or <jats:italic>in vivo<\/jats:italic> in either muscle. In conclusion, in the soleus, <jats:italic>in vivo<\/jats:italic> exercise induces a rise in intracellular Na<jats:sup>+<\/jats:sup>, which reflects the excitation\u2010induced increase in Na<jats:sup>+<\/jats:sup> influx and leads to augmented Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> pump activity without apparent change in Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup> pump capacity.<\/jats:p>","DOI":"10.1113\/expphysiol.2008.042457","type":"journal-article","created":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:44:48Z","timestamp":1214613888000},"page":"1249-1262","source":"Crossref","is-referenced-by-count":30,"title":["Analysis of exercise\u2010induced Na<sup>+<\/sup>\u2013K<sup>+<\/sup> exchange in rat skeletal muscle <i>in vivo<\/i>"],"prefix":"10.1113","volume":"93","author":[{"given":"K. T.","family":"Murphy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. 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