{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:43:16Z","timestamp":1773661396311,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,8,14]],"date-time":"2008-08-14T00:00:00Z","timestamp":1218672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p>Regular exercise is beneficial to cardiovascular health. We tested whether mild voluntary exercise training modifies key myocardial parameters [ventricular mass, intracellular calcium ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) handling and the response to \u03b2\u2010adrenoceptor (\u03b2\u2010AR) stimulation] in a manner distinct from that reported for beneficial, intensive training and pathological hypertrophic stimuli. Female rats performed voluntary wheel\u2010running exercise for 6\u20137 weeks. The mRNA expression of target proteins was measured in left ventricular tissue using real\u2010time reverse transcriptase\u2010polymerase chain reaction. Simultaneous measurement of cell shortening and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transients were made in single left ventricular myocytes and the inotropic response to \u03b2<jats:sub>1<\/jats:sub>\u2010 and \u03b2<jats:sub>2<\/jats:sub>\u2010AR stimulation was measured. Voluntary exercise training resulted in cardiac hypertrophy, the heart weight to body weight ratio being significantly greater in trained compared with sedentary animals. However, voluntary exercise caused no significant alteration in the size or time course of myocyte shortening and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transients or in the mRNA levels of key proteins that regulate Ca<jats:sup>2+<\/jats:sup> handling. The positive inotropic response to \u03b2<jats:sub>1<\/jats:sub>\u2010AR stimulation and the level of \u03b2<jats:sub>1<\/jats:sub>\u2010AR mRNA were unaltered by voluntary exercise but both mRNA levels and inotropic response to \u03b2<jats:sub>2<\/jats:sub>\u2010AR stimulation were significantly reduced in trained animals. The \u03b2<jats:sub>2<\/jats:sub>\u2010AR inotropic response was restored by exposure to pertussis toxin. We propose that in contrast to pathological stimuli and to beneficial, intense exercise training, modulation of Ca<jats:sup>2+<\/jats:sup> handling is not a major adaptive mechanism in the response to mild voluntary exercise. In addition, and in a reversal of the situation seen in heart failure, voluntary exercise training maintains the \u03b2<jats:sub>1<\/jats:sub>\u2010AR response but reduces the \u03b2<jats:sub>2<\/jats:sub>\u2010AR response. Therefore, although voluntary exercise induces cardiac hypertrophy, there are distinct differences between its effects on key myocardial regulatory mechanisms and those of hypertrophic stimuli that eventually cause cardiac decompensation.<\/jats:p>","DOI":"10.1113\/expphysiol.2008.042598","type":"journal-article","created":{"date-parts":[[2008,5,17]],"date-time":"2008-05-17T00:45:07Z","timestamp":1210985107000},"page":"1065-1075","source":"Crossref","is-referenced-by-count":21,"title":["Voluntary exercise\u2010induced changes in \u03b2<sub>2<\/sub>\u2010adrenoceptor signalling in rat ventricular myocytes"],"prefix":"10.1113","volume":"93","author":[{"given":"Rachel","family":"Stones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Natali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolf","family":"Billeter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ed","family":"White","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,8,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"H2084","DOI":"10.1152\/ajpheart.1995.268.5.H2084","article-title":"Endothelin\u22121 and its binding sites are upregulated in pressure overload cardiac hypertrophy","volume":"268","author":"Arai M","year":"1995","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.MSS.0000128143.93407.39"},{"key":"e_1_2_5_4_1","first-page":"182","article-title":"Brain natriuretic peptide in diagnosis and treatment of heart failure","volume":"49","author":"Bhatia V","year":"2003","journal-title":"J Postgrad Med"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"H805","DOI":"10.1152\/ajpheart.1993.264.3.H805","article-title":"Effects of exercise on myocardial adenylate cyclase and Gi\u03b1 expression in senescence","volume":"264","author":"Bohm M","year":"1993","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.59.3.297"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/27.7.1268"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00706.2007"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050728"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.94.4.857"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5313.800"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.99.4.546"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118090"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.06.013"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2741\/2685"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.03.010"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.132.5.8477647"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200001)182:1<1::AID-JCP1>3.0.CO;2-V"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg227"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-9164(97)90045-2"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.04.013"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300129-MCP200"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310405200"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1934654100"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0033-0620(05)80019-2"},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1152\/jappl.1982.52.5.1133","article-title":"Effect of training on \u03b2 adrenergic receptor number in rat heart","volume":"52","author":"Moore RL","year":"1982","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.4.R1321"},{"key":"e_1_2_5_29_1","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1242\/jeb.204.6.1191","article-title":"Regional effects of voluntary exercise on cell size and contraction\u2010frequency responses in rat cardiac myocytes","volume":"204","author":"Natali AJ","year":"2001","journal-title":"J Exp Biol"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013415"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-200838010-00006"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(96)00040-8"},{"key":"e_1_2_5_33_1","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1152\/jappl.1991.70.4.1633","article-title":"\u03b2\u2010Adrenoceptor adenylate cyclase system adaptation to physical training in rat ventricular tissue","volume":"70","author":"Plourde G","year":"1991","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_34_1","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1152\/jappl.1989.66.3.1250","article-title":"Variations in running activity and enzymatic adaptations in voluntary running rats","volume":"66","author":"Rodnick KJ","year":"1992","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.84.1.177"},{"key":"e_1_2_5_36_1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1152\/jappl.1994.77.2.737","article-title":"Influence of exercise training on myocardial \u03b2\u2010adrenergic signal transduction: differential regulation with age","volume":"77","author":"Scarpace PJ","year":"1994","journal-title":"J Appl Physiol"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.D200002-MCP200"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.1.57"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.9.5783"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc3401.093154"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-007-0237-z"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/16.9.530"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01906747"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(95)91911-2"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(90)90051-R"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/14.3.177"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(84)80611-2"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(01)00210-3"},{"key":"e_1_2_5_49_1","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1096\/fj.01-0792fje","article-title":"Prolonged unloading of rat soleus muscle causes distinct and coordinated adaptations of the gene profile","volume":"16","author":"Wittwer M","year":"2002","journal-title":"FASEB J"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.1.43"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.73.2.286"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000087595.17277.73"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.6.3221"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.75.3.1334"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.2008.042598","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.2008.042598","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.2008.042598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T23:07:17Z","timestamp":1697065637000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.2008.042598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,14]]},"references-count":53,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1113\/expphysiol.2008.042598"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.2008.042598","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,14]]}}}