{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:50:03Z","timestamp":1774975803554,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,6,15]],"date-time":"2009-06-15T00:00:00Z","timestamp":1245024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:p>Patch\u2010clamp and fluorescence measurements of cytoplasmic Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) were performed to directly detect extracellular Ca<jats:sup>2+<\/jats:sup>entry into cultured parathyroid cells from patients with secondary hyperparathyroidism. Cells loaded with fluo\u20103 AM or fluo\u20104 AM showed a transient increase in fluorescence (Ca<jats:sup>2+<\/jats:sup>transient) following 10 s exposure to 150 m<jats:sc>m<\/jats:sc>K<jats:sup>+<\/jats:sup>solution in the presence of millimolar concentrations of external Ca<jats:sup>2+<\/jats:sup>. The Ca<jats:sup>2+<\/jats:sup>transient was completely inactivated after 30\u201340 s exposure to the high\u2010K<jats:sup>+<\/jats:sup>solution, was reduced by dihydropyridine antagonists and was enhanced by FPL\u201064176, an L\u2010type Ca<jats:sup>2+<\/jats:sup>channel agonist. The electrophysiological and pharmacological properties of the whole\u2010cell Ca<jats:sup>2+<\/jats:sup>and Ba<jats:sup>2+<\/jats:sup>currents were similar to those of L\u2010type Ca<jats:sup>2+<\/jats:sup>channels. The Ca<jats:sup>2+<\/jats:sup>transients induced by 10 s exposure to 3.0 m<jats:sc>m<\/jats:sc>extracellular Ca<jats:sup>2+<\/jats:sup>concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>) were inhibited by dihydropyridine antagonists and were partly inactivated following 30\u201340 s exposure to the high\u2010K<jats:sup>+<\/jats:sup>solution. These results demonstrate, for the first time, that human parathyroid cells express L\u2010type\u2010like Ca<jats:sup>2+<\/jats:sup>channels that are possibly involved in the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>o<\/jats:sub>\u2010induced change in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. This Ca<jats:sup>2+<\/jats:sup>entry system might provide a compensatory pathway for the negative feedback regulation of parathyroid hormone secretion, especially in hyperplastic conditions in which the Ca<jats:sup>2+<\/jats:sup>\u2010sensing receptor is poorly expressed.<\/jats:p>","DOI":"10.1113\/expphysiol.2009.046813","type":"journal-article","created":{"date-parts":[[2009,4,4]],"date-time":"2009-04-04T01:50:30Z","timestamp":1238809830000},"page":"847-855","source":"Crossref","is-referenced-by-count":6,"title":["Dihydropyridine\u2010 and voltage\u2010sensitive Ca<sup>2+<\/sup>entry in human parathyroid 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