{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:39:30Z","timestamp":1791092370994,"version":"4.1.0"},"reference-count":77,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,12,15]],"date-time":"2009-12-15T00:00:00Z","timestamp":1260835200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>The enzymes required for aldosterone synthesis from cholesterol are expressed in rat and human brains. The hypertension of Dahl salt\u2010sensitive (SS) rats is mitigated by the intracerebroventricular (<jats:sc>i.c.v<\/jats:sc>.) infusion of antagonists of the mineralocorticoid receptor (MR) and downstream effectors of mineralocorticoid action, as well as ablations of brain areas that also abrogate mineralocorticoid\u2013salt excess hypertension in normotensive rats. We used real time RT\u2010PCR to measure mRNA of aldosterone synthase and 11\u03b2\u2010hydroxylase, the requisite enzymes for the last step in the synthesis of aldosterone and corticosterone, respectively, MR and the determinants of MR ligand specificity, 11\u03b2\u2010hydroxysteroid dehydrogenase types 1 and 2 (11\u03b2\u2010HSD1&amp;2) and hexose\u20106\u2010phosphate dehydrogenase (H6PDH). A combination of extraction and ELISA was used to measure aldosterone concentrations in tissue and urine of SS and Sprague\u2013Dawley (SD) rats. Aldosterone synthase mRNA expression was higher in the brains and lower in the adrenal glands of SS compared with SD rats. The amounts of mRNA for MR, 11\u03b2\u2010hydroxylase, 11\u03b2\u2010HSD1&amp;2 and H6PD were similar. Aldosterone concentrations were greater in brains of SS than SD rats, yet, in keeping with the literature, the circulating and total aldosterone production of aldosterone in SS rats were not. The selective inhibitor of aldosterone synthase, FAD286, was infused <jats:sc>i.c.v<\/jats:sc>. or subcutaneously in a cross\u2010over blood pressure study in hypertensive SS rats further challenged by a high\u2010salt diet. The <jats:sc>i.c.v<\/jats:sc>. infusion of FAD286, at a dose that had no effect systemically, significantly and reversibly lowered blood pressure in SS rats. Aldosterone synthesis in brains of SS rats is greater than in SD rats and is important in the genesis of their salt\u2010sensitive hypertension.<\/jats:p>","DOI":"10.1113\/expphysiol.2009.048900","type":"journal-article","created":{"date-parts":[[2009,10,17]],"date-time":"2009-10-17T00:45:45Z","timestamp":1255740345000},"page":"120-130","source":"Crossref","is-referenced-by-count":76,"title":["Aldosterone synthesis in the brain contributes to Dahl salt\u2010sensitive rat hypertension"],"prefix":"10.1113","volume":"95","author":[{"given":"Elise P.","family":"Gomez\u2010Sanchez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara M.","family":"Gomez\u2010Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Plonczynski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso E.","family":"Gomez\u2010Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,12,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1370445"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.06.065"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.8.12.1149"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404159200"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01963313"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-3057(98)00071-9"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.18.5_Suppl.III7"},{"key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1001\/archinte.1972.03650060041007","article-title":"The syndrome of essential hypertension and suppressed plasma renin activity. Normalization of blood pressure with spironolactone","volume":"130","author":"Carey RM","year":"1972","journal-title":"Arch Intern Med"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0493-346"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.28.16555"},{"key":"e_1_2_5_12_1","volume-title":"Physiology of the CSF and Blood\u2010Brain Barriers","author":"Davson H","year":"1996"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00971.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)90834-X"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.521625"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20808"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.10.044"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199305000-00002"},{"issue":"5","key":"e_1_2_5_19_1","first-page":"S227","article-title":"Urinary mineralocorticoids in genetically hypertensive rats of the Lyon strain","volume":"5","author":"Gomez\u2010Sanchez CE","year":"1987","journal-title":"J Hypertension"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-0860"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(87)90097-3"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-001-0049-z"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-99-2-567"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/07435809609043732"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.138.8.3369"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-2-819"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1994.tb39272.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00041552-200403000-00007"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-0295"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00355.2004"},{"key":"e_1_2_5_31_1","doi-asserted-by":"crossref","first-page":"E96","DOI":"10.1152\/ajpendo.1992.262.1.E96","article-title":"Central mineralocorticoid receptor antagonism blocks hypertension in Dahl S\/JR rats","volume":"262","author":"Gomez\u2010Sanchez EP","year":"1992","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"e_1_2_5_32_1","doi-asserted-by":"crossref","first-page":"E482","DOI":"10.1152\/ajpendo.1990.258.3.E482","article-title":"Intracerebroventricular infusion of RU28318 blocks aldosterone\u2010salt hypertension","volume":"258","author":"Gomez\u2010Sanchez EP","year":"1990","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00409.2002"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-3-1110"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(91)90035-T"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(91)90061-9"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1994.267.5.E754"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1995.269.3.H1044"},{"key":"e_1_2_5_39_1","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1210\/endo.138.12.5669","article-title":"Editorial: First there was one, then two\u2026 why more 11\u03b2\u2010hydroxysteroid dehydrogenases?","volume":"138","author":"Gomez\u2010Sanchez EP","year":"1997","journal-title":"Endocrinology"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.33.6.1369"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953059"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0328"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00441.2004"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1990.258.4.E649"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs061053s"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00024.2006"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00840.2004"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00126.2004"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00651.2004"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.90352.2008"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn222"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.90903.2008"},{"key":"e_1_2_5_53_1","doi-asserted-by":"crossref","first-page":"R166","DOI":"10.1152\/ajpregu.1988.255.1.R166","article-title":"Central interactions between aldosterone and vasopressin on cardiovascular system","volume":"255","author":"Janiak P","year":"1988","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"e_1_2_5_54_1","doi-asserted-by":"crossref","first-page":"R1025","DOI":"10.1152\/ajpregu.1990.259.5.R1025","article-title":"Role of central mineralocorticoid binding sites in development of hypertension","volume":"259","author":"Janiak PC","year":"1990","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-94-6-1541"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00043.2001"},{"key":"e_1_2_5_57_1","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1001\/archinte.1977.03630200048015","article-title":"Relationship of plasma renin activity and aldosterone levels with hemodynamic functions in essential hypertension","volume":"137","author":"London GM","year":"1977","journal-title":"Arch Intern Med"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0240321"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0290319"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.17.1_Suppl.I86"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.78.5.1003"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1993.tb09533.x"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2003.10.009"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.3109\/07435807809073634"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-117-6-2505"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.137.3.1123"},{"key":"e_1_2_5_67_1","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1210\/endo.142.4.8114","article-title":"Minireview: 11\u03b2\u2010hydroxysteroid dehydrogenase type 1\u2010 a tissue\u2010specific amplifier of glucocorticoid action","volume":"142","author":"Seckl JR","year":"2001","journal-title":"Endocrinology"},{"key":"e_1_2_5_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(87)91014-2"},{"key":"e_1_2_5_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(95)00140-N"},{"key":"e_1_2_5_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/1.3.45S"},{"key":"e_1_2_5_71_1","doi-asserted-by":"crossref","first-page":"H48","DOI":"10.1152\/ajpheart.1979.236.1.H48","article-title":"Prevention of salt\u2010induced hypertension in Dahl strain by 6\u2010hydroxydopamine","volume":"236","author":"Takeshita A","year":"1979","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"e_1_2_5_72_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2003-0031"},{"key":"e_1_2_5_73_1","first-page":"S19","article-title":"Transcardiac gradient of aldosterone before and after spironolactone in patients with congestive heart failure","volume":"41","author":"Tsutamoto T","year":"2003","journal-title":"J Cardiovasc Pharmacol"},{"key":"e_1_2_5_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(00)00796-8"},{"key":"e_1_2_5_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02245882"},{"key":"e_1_2_5_76_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0667"},{"key":"e_1_2_5_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00041-2"},{"key":"e_1_2_5_78_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.099796"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.2009.048900","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.2009.048900","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.2009.048900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T14:14:37Z","timestamp":1698761677000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.2009.048900"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,15]]},"references-count":77,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1113\/expphysiol.2009.048900"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.2009.048900","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,15]]}}}