{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:17:57Z","timestamp":1792225077316,"version":"4.4.0"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,6,11]],"date-time":"2010-06-11T00:00:00Z","timestamp":1276214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:p><jats:bold>The renin\u2013angiotensin system is activated in the early phase of two\u2010kidney, one\u2010clip (2K\u20101C) hypertension. The paraventricular nucleus (PVN) integrates inputs regulating sympathetic outflow. The PVN receives inputs from plasma angiotensin II via projections from circumventricular organs and from renal afferent nerves transmitted via the nucleus tractus solitarii. Nitric oxide within the PVN may exert a sympathoinhibitory effect. These studies tested whether decreasing endogenous nitric oxide by introducing dominant negative (DN) constructs for neuronal nitric oxide synthase (nNOS) into PVN chronically augments hypertension and\/or modulates baroreflex function. Male 6\u2010week\u2010old Sprague\u2013Dawley rats underwent sham surgery or right renal artery clipping and placement of radiotelemetry transmitters. One week later, the PVN was injected bilaterally with 250 nl artificial cerebrospinal fluid containing 250 ng \u03bcl<jats:sup>\u22121<\/jats:sup> of RSV \u03b2\u2010galactosidase (\u03b2\u2010Gal), cytomegalovirus (CMV) wild\u2010type (WT nNOS), or respiratory syncytial virus (RSV) haeme domain or RSV haemeRedF (DN nNOS). Haemodynamics were monitored for 5 weeks. Then left renal nerve electrodes were placed, and 2 days later the rats underwent baroreflex testing in the conscious state. The rise in mean arterial pressure (MAP) was significantly potentiated in the DN nNOS 2K\u20101C group beyond 15 days after PVN injection. By day 35, MAP in the 2K\u20101C groups was 152 \u00b1 6.3 (\u03b2\u2010Gal), 155.1 \u00b1 6.6 (WT nNOS) and 179 \u00b1 5.4 mmHg (DN nNOS; <jats:italic>P<\/jats:italic> &lt; 0.01 <jats:italic>versus<\/jats:italic> all other groups). Sham\u2010clipped rats remained normotensive. All groups displayed progressive bradycardia over time that was attenuated in the DN nNOS 2K\u20101C group. Baroreflex curves shifted to higher pressures, and baroreflex sensitivity of heart rate was diminished to a similar extent in all groups of 2K\u20101C rats. The baroreflex response of renal sympathetic nerve activity was preserved. The PVN tissue from DN nNOS rats had decreased dimerization of nNOS and generation of total nitric oxide. These findings indicate that chronic interference of nNOS dimerization required for generation of nitric oxide within the PVN potentiates the increase of blood pressure by modulating the sympathoexcitation that accompanies renovascular hypertension.<\/jats:bold><\/jats:p>","DOI":"10.1113\/expphysiol.2009.051789","type":"journal-article","created":{"date-parts":[[2010,5,22]],"date-time":"2010-05-22T00:14:19Z","timestamp":1274487259000},"page":"845-857","source":"Crossref","is-referenced-by-count":40,"title":["Neuronal nitric oxide synthase within paraventricular nucleus: blood pressure and baroreflex in two\u2010kidney, one\u2010clip hypertensive rats"],"prefix":"10.1113","volume":"95","author":[{"given":"Noreen 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