{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T21:38:45Z","timestamp":1761946725357},"reference-count":26,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2010,9,28]],"date-time":"2010-09-28T00:00:00Z","timestamp":1285632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p><jats:bold>The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min \u2018Schmidt\u2019 and 40 min \u2018Burge\u2019 methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (<jats:italic>t<\/jats:italic><jats:sub>mix<\/jats:sub>), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash\u2010out was assessed on calculated haemoglobin mass (Hb<jats:sub>mass<\/jats:sub>). Arterial (<jats:italic>a<\/jats:italic>), muscle venous (<jats:italic>vm<\/jats:italic>) and capillary samples (<jats:italic>c<\/jats:italic>) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash\u2010out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after <jats:italic>t<\/jats:italic><jats:sub>mix<\/jats:sub>, and the rate of CO wash\u2010out. In both methods, HbCO<jats:italic>a<\/jats:italic> and HbCO<jats:italic>c<\/jats:italic> followed a near\u2010identical time course, peaking within the first 2 min and decreasing thereafter. The HbCO<jats:italic>vm<\/jats:italic> increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (<jats:italic>P<\/jats:italic> &lt; 0.01). The HbCO<jats:italic>a<\/jats:italic> peaked significantly higher in the Schmidt method (<jats:italic>P<\/jats:italic>= 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash\u2010out was 0.25 \u00b1 0.13 ml min<jats:sup>\u22121<\/jats:sup> in the Schmidt and 0.25 \u00b1 0.16 ml min<jats:sup>\u22121<\/jats:sup> in the Burge method. The rate of CO diffusion to Mb was 0.22 \u00b1 0.11 and 0.16 \u00b1 0.13 ml min<jats:sup>\u22121<\/jats:sup> (<jats:italic>P<\/jats:italic>= 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCO<jats:italic>a<\/jats:italic> uptake but does not greatly shorten <jats:italic>t<\/jats:italic><jats:sub>mix<\/jats:sub> or influence rates of CO wash\u2010out and flux to Mb. The calculated Hb<jats:sub>mass<\/jats:sub> depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within\u2010subject alterations of CO exhalation and CO flux to Mb.<\/jats:bold><\/jats:p>","DOI":"10.1113\/expphysiol.2010.054031","type":"journal-article","created":{"date-parts":[[2010,9,4]],"date-time":"2010-09-04T00:46:00Z","timestamp":1283561160000},"page":"1156-1166","source":"Crossref","is-referenced-by-count":38,"title":["Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing"],"prefix":"10.1113","volume":"95","author":[{"given":"Laura A.","family":"Garvican","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline M.","family":"Burge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda J.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally A.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David T.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Gore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,9,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.76.4.1739"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI102551"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00217.2003"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00512.2005"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1995.79.2.623"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00298.2010"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-009-1027-4"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00719.2007"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/JSM.0b013e3181ddcd5b"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.mss.0000222848.35004.41"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00505.2005"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.2004.03598.x"},{"key":"e_1_2_5_15_1","first-page":"1450","article-title":"Mesures du volume du sang contenu dans l\u2019organisme d\u2019un mammifere vivant","volume":"94","author":"Grehant M","year":"1882","journal-title":"C. 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