{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:01:31Z","timestamp":1784307691716,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,7,25]],"date-time":"2014-07-25T00:00:00Z","timestamp":1406246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>In humans, \u2018Western\u2010style\u2019 diet is characterized by high levels of both saturated fats and fructose. Lipid oversupply to the liver typical of high\u2010fat diets could be exacerbated by the coexistence of high levels of fat and fructose in the diet, thus accelerating the development of metabolic deregulation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Short\u2010term consumption of a Western diet, rich in saturated fats and fructose, is more conducive to the development of liver steatosis and deleterious to glucose homeostasis than a high\u2010fat diet. This result points to the harmful effect of adding fructose to the usual Western, high\u2010fat diet.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>The purpose of the present study was to examine the short\u2010term effect of high\u2010fat or high\u2010fat\u2013high\u2010fructose feeding on hepatic lipid metabolism and mitochondrial function in adult sedentary rats. Adult male rats were fed a high\u2010fat or high\u2010fat\u2013high\u2010fructose diet for 2\u00a0weeks. Body and liver composition, hepatic steatosis, plasma lipid profile and hepatic insulin sensitivity, together with whole\u2010body and hepatic <jats:italic>de novo<\/jats:italic> lipogenesis, were assessed. Hepatic mitochondrial mass, functionality, oxidative stress and antioxidant defense were also measured. Rats fed the high\u2010fat\u2013high\u2010fructose diet exhibited significantly higher plasma triglycerides, non\u2010esterified fatty acids, insulin and indexes of hepatic insulin resistance compared with rats fed a low\u2010fat or a high\u2010fat diet. Hepatic triglycerides and ceramide, as well as the degree of steatosis and necrosis, were significantly higher, while liver p\u2010Akt was significantly lower, in rats fed high\u2010fat\u2013high\u2010fructose diet than in rats fed high\u2010fat diet. A significant increase in non\u2010protein respiratory quotient and hepatic fatty acid synthase and stearoyl CoA desaturase activity was found in rats fed the high\u2010fat\u2013high\u2010fructose diet compared with those fed the high\u2010fat diet. Significantly lower mitochondrial oxidative capacity but significantly higher oxidative stress was found in rats fed high\u2010fat and high\u2010fat\u2013high\u2010fructose diets compared with rats fed low\u2010fat diet, while mitochondrial mass significantly increased only in rats fed high\u2010fat\u2013high\u2010fructose diet. In conclusion, short\u2010term consumption of a Western diet, rich in saturated fats and fructose, is more conducive to the development of liver steatosis and deleterious to glucose homeostasis than a high\u2010fat diet.<\/jats:p><\/jats:sec>","DOI":"10.1113\/expphysiol.2014.079632","type":"journal-article","created":{"date-parts":[[2014,6,28]],"date-time":"2014-06-28T04:42:35Z","timestamp":1403930555000},"page":"1203-1213","source":"Crossref","is-referenced-by-count":54,"title":["Fructose supplementation worsens the deleterious effects of short\u2010term high\u2010fat feeding on hepatic steatosis and lipid metabolism in adult rats"],"prefix":"10.1113","volume":"99","author":[{"given":"Raffaella","family":"Crescenzo","sequence":"first","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Bianco","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Coppola","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arianna","family":"Mazzoli","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margherita","family":"Tussellino","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Carotenuto","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Liverini","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Iossa","sequence":"additional","affiliation":[{"name":"Department of Biology University of Naples  Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc06-1519"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90207.2008"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0b013e32834ad966"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-011-1147-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2011.04.007"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114513001566"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114511005903"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-012-0356-y"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2008.10"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.03.004"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2006.06.002"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717-95022013000100003"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05013-8"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)49317-2"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2003000968"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-3977fje"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0803636"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2011.10.008"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/420754"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2007.11.021"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1042\/bj2790303","article-title":"Increased gene expression of lipogenic enzymes and glucose transporter in white adipose tissue of suckling and weaned obese Zucker rats","volume":"279","author":"Penicaud L","year":"1991","journal-title":"Biochem J"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.27249"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.12.006"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030816"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(74)90210-3"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2007.tb00322.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.11.4565"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.2014.079632","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.2014.079632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T08:58:32Z","timestamp":1694681912000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.2014.079632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,25]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1113\/expphysiol.2014.079632"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.2014.079632","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,25]]}}}