{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:25:00Z","timestamp":1792787100367,"version":"4.5.0"},"reference-count":74,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2015,7,20]],"date-time":"2015-07-20T00:00:00Z","timestamp":1437350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guglietti Family Foundation"},{"name":"BioGaia AB"},{"name":"Natural Sciences and Research Engineering Council (NSERC)","award":["RGPIN-2014-05517"],"award-info":[{"award-number":["RGPIN-2014-05517"]}]},{"name":"Father Sean O'Sullivan Research Centre Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Certain probiotic bacteria have been shown to reduce distension\u2010dependent gut pain, but the mechanisms involved remain obscure.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Live luminal\n                            <jats:italic>Lactobacillus reuteri<\/jats:italic>\n                            (DSM 17938) and its conditioned medium dose dependently reduced jejunal spinal nerve firing evoked by distension or capsaicin, and 80% of this response was blocked by a specific TRPV1 channel antagonist or in TRPV1 knockout mice.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>The specificity of DSM action on TRPV1 was further confirmed by its inhibition of capsaicin\u2010induced intracellular calcium increases in dorsal root ganglion neurons. Another lactobacillus with ability to reduce gut pain did not modify this response.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Prior feeding of rats with DSM inhibited the bradycardia induced by painful gastric distension.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>These results offer a system for the screening of new and improved candidate bacteria that may be useful as novel therapeutic adjuncts in gut pain.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Certain bacteria exert visceral antinociceptive activity, but the mechanisms involved are not determined.\n                      <jats:italic>Lactobacillus reuteri<\/jats:italic>\n                      DSM 17938 was examined since it may be antinociceptive in children. Since transient receptor potential vanilloid 1 (TRPV1) channel activity may mediate nociceptive signals, we hypothesized that TRPV1 current is inhibited by DSM. We tested this by examining the effect of DSM on the firing frequency of spinal nerve fibres in murine jejunal mesenteric nerve bundles following serosal application of capsaicin. We also measured the effects of DSM on capsaicin\u2010evoked increase in intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      or ionic current in dorsal root ganglion (DRG) neurons. Furthermore, we tested the\n                      <jats:italic>in vivo<\/jats:italic>\n                      antinociceptive effects of oral DSM on gastric distension in rats. Live DSM reduced the response of capsaicin\u2010 and distension\u2010evoked firing of spinal nerve action potentials (238\u00a0\u00b1\u00a027.5%\u00a0\n                      <jats:italic>vs<\/jats:italic>\n                      . 129\u00a0\u00b1\u00a017%). DSM also reduced the capsaicin\u2010evoked TRPV1 ionic current in DRG neuronal primary culture from 83\u00a0\u00b1\u00a011% to 41\u00a0\u00b1\u00a08% of the initial response to capsaicin only. Another lactobacillus (\n                      <jats:italic>Lactobacillus rhamnosus<\/jats:italic>\n                      JB\u20101) with known visceral anti\u2010nociceptive activity did not have these effects. DSM also inhibited capsaicin\u2010evoked Ca\n                      <jats:sup>2+<\/jats:sup>\n                      increase in DRG neurons; an increase in Ca\n                      <jats:sup>2+<\/jats:sup>\n                      fluorescence intensity ratio of 2.36\u00a0\u00b1\u00a00.31 evoked by capsaicin was reduced to 1.25\u00a0\u00b1\u00a00.04. DSM releasable products (conditioned medium) mimicked DSM inhibition of capsaicin\u2010evoked excitability. The TRPV1 antagonist 6\u2010iodonordihydrocapsaicin or the use of TRPV1 knock\u2010out mice revealed that TRPV1 channels mediate about 80% of the inhibitory effect of DSM on mesenteric nerve response to high intensity gut distension. Finally, feeding with DSM inhibited perception in rats of painful gastric distension. Our results identify a specific target channel for a probiotic with potential therapeutic properties.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp270229","type":"journal-article","created":{"date-parts":[[2015,6,18]],"date-time":"2015-06-18T06:03:03Z","timestamp":1434607383000},"page":"3943-3957","source":"Crossref","is-referenced-by-count":124,"title":["The TRPV1 channel in rodents is a major target for antinociceptive effect of the probiotic\n                    <i>Lactobacillus reuteri<\/i>\n                    DSM 17938"],"prefix":"10.1113","volume":"593","author":[{"given":"Azucena","family":"Perez\u2010Burgos","sequence":"first","affiliation":[{"name":"McMaster Brain\u2010Body Institute St Joseph's Healthcare, Hamilton  50 Charlton Avenue East Hamilton Ontario Canada L8N 4A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Farncombe Family Digestive Health Research Institute McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen\u2010Anne","family":"McVey Neufeld","sequence":"additional","affiliation":[{"name":"McMaster Brain\u2010Body Institute St Joseph's Healthcare, Hamilton  50 Charlton Avenue East Hamilton Ontario Canada L8N 4A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Kang","family":"Mao","sequence":"additional","affiliation":[{"name":"McMaster Brain\u2010Body Institute St Joseph's Healthcare, Hamilton  50 Charlton Avenue East Hamilton Ontario Canada L8N 4A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Ahmadzai","sequence":"additional","affiliation":[{"name":"Firestone Institute for Respiratory Health, St Joseph's Hospital, Department of Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke J.","family":"Janssen","sequence":"additional","affiliation":[{"name":"Firestone Institute for Respiratory Health, St Joseph's Hospital, Department of Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Stanisz","sequence":"additional","affiliation":[{"name":"McMaster Brain\u2010Body Institute St Joseph's Healthcare, Hamilton  50 Charlton Avenue East Hamilton Ontario Canada L8N 4A6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Bienenstock","sequence":"additional","affiliation":[{"name":"McMaster Brain\u2010Body Institute St Joseph's Healthcare, Hamilton  50 Charlton Avenue East Hamilton Ontario Canada L8N 4A6"},{"name":"Department of Pathology and Molecular Medicine McMaster University  Hamilton Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang A.","family":"Kunze","sequence":"additional","affiliation":[{"name":"McMaster 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