{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:36:05Z","timestamp":1790267765820,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministry of Education, Science and Technological Development of the Republic of Serbia","doi-asserted-by":"crossref","award":["173054, 173055"],"award-info":[{"award-number":["173054, 173055"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>White to brown adipose tissue conversion and thermogenesis can be ignited by different conditions or agents and its sustainability over the long term is still unclear.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Browning of rat retroperitoneal white adipose tissue (rpWAT) during cold acclimation involves two temporally apparent components: (1) a predominant non\u2010selective browning of most adipocytes and an initial sharp but transient induction of uncoupling protein 1, peroxisome proliferator\u2010activated receptor (PPAR) coactivator\u20101\u03b1, PPAR\u03b3 and PPAR\u03b1 expression, and (2) the subsistence of relatively few thermogenically competent adipocytes after 45\u00a0days of cold acclimation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The different behaviours of two rpWAT beige\/brown adipocyte subsets control temporal aspects of the browning process, and thus regulation of both components may influence body weight and the potential successfulness of anti\u2010obesity therapies.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Conversion of white into brown adipose tissue may have important implications in obesity resistance and treatment. Several browning agents or conditions ignite thermogenesis in white adipose tissue (WAT). To reveal the capacity of WAT to function in a brownish\/burning mode over the long term, we investigated the progression of the rat retroperitoneal WAT (rpWAT) browning during 45\u00a0days of cold acclimation. During the early stages of cold acclimation, the majority of rpWAT adipocytes underwent multilocularization and thermogenic\u2010profile induction, as demonstrated by the presence of a multitude of uncoupling protein 1 (UCP1)\u2010immunopositive paucilocular adipocytes containing peroxisome proliferator\u2010activated receptor (PPAR) coactivator\u20101\u03b1 (PGC\u20101\u03b1) and PR domain\u2010containing 16 (PRDM16) in their nuclei. After 45\u00a0days, all adipocytes remained PRDM16 immunopositive, but only a few multilocular adipocytes rich in mitochondria remained UCP1\/PGC\u20101\u03b1 immunopositive. Molecular evidence showed that thermogenic recruitment of rpWAT occurred following cold exposure, but returned to starting levels after cold acclimation. Compared with controls (22\u00a0\u00b1\u00a01\u00b0C), levels of UCP1 mRNA increased in parallel with PPAR\u03b3 (PPAR\u03b1 from days 1 to 7 and PGC\u20101\u03b1 on day 1). Transcriptional recruitment of rpWAT was followed by an increase in UCP1 protein content (from days 1 to 21). Results clearly showed that most of the adipocytes within rpWAT underwent transient brown\u2010fat\u2010like thermogenic recruitment upon stimulation, but only a minority of cells retained a brown adipose tissue\u2010like phenotype after the attainment of cold acclimation. Therefore, browning of WAT is dependent on both maintaining the thermogenic response and retaining enough brown\u2010like thermogenically competent adipocytes in the long\u2010term. Both aspects of browning could be important for long\u2010term energy homeostasis and body\u2010weight regulation.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp270805","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T17:36:06Z","timestamp":1434735366000},"page":"3267-3280","source":"Crossref","is-referenced-by-count":66,"title":["Two key temporally distinguishable molecular and cellular components of white adipose tissue browning during cold acclimation"],"prefix":"10.1113","volume":"593","author":[{"given":"Aleksandra","family":"Jankovic","sequence":"first","affiliation":[{"name":"Department of Physiology, Institute for Biological Research \u2018Sinisa Stankovic\u2019 University of Belgrade  Bulevar despota Stefana 142 11060 Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Golic","sequence":"additional","affiliation":[{"name":"Faculty of Biology, Center for Electron Microscopy University of Belgrade  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