{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:43:26Z","timestamp":1791272606708,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T00:00:00Z","timestamp":1453248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2016,3,15]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Endothelin\u20101 (ET\u20101) is upregulated upon intermittent hypoxia and has lipolytic effects.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Intermittent hypoxia induces adipose tissue lipolysis that may be mediated by ET\u20101.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the present study, we show that ET\u20101 is involved in adipose tissue remodelling induced by intermittent hypoxia and that phosphorylation of hormone\u2010sensitive lipase could be one mechanism mediating this effect.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We also show that ET\u20101 upregulates its own and its type A endothelin receptor expression, possibly leading to an autoactivatory loop.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results help us better understand the mechanisms of dyslipidaemia in disorders associated with intermittent hypoxia, such as obstructive sleep apnoea.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Obstructive sleep apnoea syndrome is characterized by repetitive episodes of upper airway collapse during sleep resulting in chronic intermittent hypoxia (IH). Obstructive sleep apnoea syndrome, through IH, promotes cardiovascular and metabolic disorders. Endothelin\u20101 (ET\u20101) secretion is upregulated by IH, and is able to modulate adipocyte metabolism. Therefore, the present study aimed to characterize the role of ET\u20101 in the metabolic consequences of IH on adipose tissue <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. Wistar rats were submitted to 14\u00a0days of IH\u2010cycles (30\u00a0s of 21% FiO<jats:sub>2<\/jats:sub>\u00a0and 30\u00a0s of 5% FiO<jats:sub>2<\/jats:sub>; 8\u00a0h\u00a0day<jats:sup>\u22121<\/jats:sup>) or normoxia (air\u2013air cycles) and were treated or not with bosentan, a dual type A and B endothelin receptor (ETA\u2010R and ETB\u2010R) antagonist. Bosentan treatment decreased plasma free fatty acid and triglyceride levels, and inhibited IH\u2010induced lipolysis in adipose tissue. Moreover, IH induced a 2\u2010fold increase in ET\u20101 transcription and ETA\u2010R expression in adipose tissue that was reversed by bosentan. In 3T3\u2010L1 adipocytes, ET\u20101 upregulated its own and its ETA\u2010R transcription and this effect was abolished by bosentan. Moreover, ET\u20101 induced glycerol release and inhibited insulin\u2010induced glucose uptake. Bosentan and BQ123 inhibited these effects. Bosentan also reversed the ET\u20101\u2010induced phosphorylation of hormone\u2010sensitive lipase (HSL) on Ser<jats:sup>660<\/jats:sup>. Finally, ET\u20101\u2010induced lipolysis and HSL phosphorylation were also observed under hypoxia. Altogether, these data suggest that ET\u20101 is involved in IH\u2010induced lipolysis in Wistar rats, and that upregulation of ET\u20101 production and ETA\u2010R expression by ET\u20101 itself under IH could amplify its effects. Moreover, ET\u20101\u2010induced lipolysis could be mediated through ETA\u2010R and activation of HSL by Ser<jats:sup>660<\/jats:sup> phosphorylation.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp271321","type":"journal-article","created":{"date-parts":[[2015,12,12]],"date-time":"2015-12-12T15:14:44Z","timestamp":1449933284000},"page":"1727-1740","source":"Crossref","is-referenced-by-count":31,"title":["Endothelin regulates intermittent hypoxia\u2010induced lipolytic remodelling of adipose tissue and phosphorylation of hormone\u2010sensitive lipase"],"prefix":"10.1113","volume":"594","author":[{"given":"Anne","family":"Brian\u00e7on\u2010Marjollet","sequence":"first","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes  HP2 Grenoble France"},{"name":"INSERM  HP2, U1042 Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Monneret","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes  HP2 Grenoble 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