{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:19:25Z","timestamp":1790813965284,"version":"4.1.0"},"reference-count":71,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,15]],"date-time":"2015-12-15T00:00:00Z","timestamp":1450137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2016,1,15]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Ribosome biogenesis is the primary determinant of translational capacity, but its regulation in skeletal muscle following acute resistance exercise is poorly understood.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Resistance exercise increases muscle protein synthesis acutely, and muscle mass with training, but the role of translational capacity in these processes is unclear.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Here, we show that acute resistance exercise activated pathways controlling translational activity and capacity through both rapamycin\u2010sensitive and \u2010insensitive mechanisms.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Transcription factor c\u2010Myc and its downstream targets, which are known to regulate ribosome biogenesis in other cell types, were upregulated after resistance exercise in a rapamycin\u2010independent manner and may play a role in determining translational capacity in skeletal muscle.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Local inhibition of myostatin was also not affected by rapamycin and may contribute to the rapamycin\u2010independent effects of resistance exercise.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>This study aimed to determine (1) the effect of acute resistance exercise on mechanisms of ribosome biogenesis, and (2) the impact of mammalian target of rapamycin on ribosome biogenesis, and muscle protein synthesis (MPS) and degradation. Female F344BN rats underwent unilateral electrical stimulation of the sciatic nerve to mimic resistance exercise in the tibialis anterior (TA) muscle. TA muscles were collected at intervals over the 36\u00a0h of exercise recovery (REx); separate groups of animals were administered rapamycin pre\u2010exercise (REx+Rapamycin). Resistance exercise led to a prolonged (6\u201336\u00a0h) elevation (30\u201350%) of MPS that was fully blocked by rapamycin at 6\u00a0h but only partially at 18\u00a0h. REx also altered pathways that regulate protein homeostasis and mRNA translation in a manner that was both rapamycin\u2010sensitive (proteasome activity; phosphorylation of S6K1 and rpS6) and rapamycin\u2010insensitive (phosphorylation of eEF2, ERK1\/2 and UBF; gene expression of the myostatin target Mighty as well as c\u2010Myc and its targets involved in ribosome biogenesis). The role of c\u2010Myc was tested <jats:italic>in vitro<\/jats:italic> using the inhibitor 10058\u2010F4, which, over time, decreased basal RNA and MPS in a dose\u2010dependent manner (correlation of RNA and MPS, <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a00.98), even though it had no effect on the acute stimulation of protein synthesis. In conclusion, acute resistance exercise stimulated rapamycin\u2010sensitive and \u2010insensitive mechanisms that regulate translation activity and capacity.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp271365","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T08:48:44Z","timestamp":1447058924000},"page":"453-468","source":"Crossref","is-referenced-by-count":151,"title":["Acute resistance exercise activates rapamycin\u2010sensitive and \u2010insensitive mechanisms that control translational activity and capacity in skeletal muscle"],"prefix":"10.1113","volume":"594","author":[{"given":"Daniel W. D.","family":"West","sequence":"first","affiliation":[{"name":"Department of Physiology &amp; Membrane Biology University of California Davis  Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie M.","family":"Baehr","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Membrane Biology University of California Davis  Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George R.","family":"Marcotte","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Membrane Biology University of California Davis  Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney M.","family":"Chason","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Physiology &amp; Behavior University of California Davis  Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Tolento","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Physiology &amp; Behavior University of California Davis  Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldrin V.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Membrane Biology University of California Davis  Davis CA USA"},{"name":"Department of Neurobiology, Physiology &amp; Behavior University of California Davis  Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue C.","family":"Bodine","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Membrane Biology University of California Davis  Davis CA USA"},{"name":"Department of Neurobiology, Physiology &amp; Behavior University of California Davis  Davis CA USA"},{"name":"VA Northern California Health Care System  Mather CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Baar","sequence":"additional","affiliation":[{"name":"Department of Physiology &amp; Membrane Biology University of California Davis  Davis CA USA"},{"name":"Department of Neurobiology, Physiology &amp; Behavior University of California Davis  Davis CA USA"},{"name":"VA Northern California Health Care System  Mather CA 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