{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:26:02Z","timestamp":1790403962674,"version":"4.1.0"},"reference-count":89,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,11,15]],"date-time":"2015-11-15T00:00:00Z","timestamp":1447545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Glucose regulates the density and function of 5\u2010HT<jats:sub>3<\/jats:sub> receptors on gastric vagal afferent neurones.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Diet\u2010induced obesity compromises the excitability and responsiveness of vagal afferents.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In this study, we assessed whether exposure to a high fat diet (HFD) compromises the glucose\u2010dependent modulation of 5\u2010HT responses in gastric vagal afferents prior to the development of obesity.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We show that HFD does not alter the response of gastric vagal afferent nerves and neurones to 5\u2010HT but attenuates the ability of glucose to amplify 5\u2010HT<jats:sub>3<\/jats:sub>\u2010induced responses.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results suggest that glucose\u2010dependent vagal afferent signalling is compromised by relatively short periods of exposure to HFD well in advance of the development of obesity or glycaemic dysregulation.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Glucose regulates the density and function of 5\u2010HT<jats:sub>3<\/jats:sub> receptors on gastric vagal afferent neurones. Since diet\u2010induced obesity attenuates the responsiveness of gastric vagal afferents to several neurohormones, the aim of the present study was to determine whether high fat diet (HFD) compromises the glucose\u2010dependent modulation of 5\u2010HT responses in gastric vagal afferents prior to the development of obesity. Rats were fed control or HFD (14% or 60% kilocalories from fat, respectively) for up to 8\u00a0weeks. Neurophysiological recordings assessed the ability of 5\u2010HT to increase anterior gastric vagal afferent nerve (VAN) activity <jats:italic>in vivo<\/jats:italic> before and after acute hyperglycaemia, while electrophysiological recordings from gastric\u2010projecting nodose neurones assessed the ability of glucose to modulate the 5\u2010HT response <jats:italic>in vitro<\/jats:italic>. Immunocytochemical studies determined alterations in the neuronal distribution of 5\u2010HT<jats:sub>3<\/jats:sub> receptors. 5\u2010HT and cholecystokinin (CCK) induced dose\u2010dependent increases in VAN activity in all rats; HFD attenuated the response to CCK, but not 5\u2010HT. The 5\u2010HT\u2010induced response was amplified by acute hyperglycaemia in control, but not HFD, rats. Similarly, although 5\u2010HT induced an inward current in both control and HFD gastric nodose neurones <jats:italic>in vitro<\/jats:italic>, the 5\u2010HT response and receptor distribution was amplified by acute hyperglycaemia only in control rats. These data suggest that, while HFD does not affect the response of gastric\u2010projecting vagal afferents to 5\u2010HT, it attenuates the ability of glucose to amplify 5\u2010HT effects. This suggests that glucose\u2010dependent vagal afferent signalling is compromised by short periods of exposure to HFD well in advance of obesity or glycaemic dysregulation.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp271558","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T03:00:09Z","timestamp":1444705209000},"page":"99-114","source":"Crossref","is-referenced-by-count":37,"title":["High fat diet attenuates glucose\u2010dependent facilitation of 5\u2010HT<sub>3<\/sub>\u2010mediated responses in rat gastric vagal afferents"],"prefix":"10.1113","volume":"594","author":[{"given":"Amanda E.","family":"Troy","sequence":"first","affiliation":[{"name":"Department of Neural and Behavioural Sciences Penn State University College of Medicine  Hershey PA 17033 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah S.","family":"Simmonds","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology Penn State University College of Medicine  Hershey PA 17033 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean D.","family":"Stocker","sequence":"additional","affiliation":[{"name":"Department of Neural and Behavioural Sciences Penn State University College of Medicine  Hershey PA 17033 USA"},{"name":"Department of Cellular and Integrative Physiology Penn State University College of Medicine  Hershey PA 17033 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsteen N.","family":"Browning","sequence":"additional","affiliation":[{"name":"Department of Neural and Behavioural Sciences Penn State University College of Medicine  Hershey PA 17033 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