{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:11:09Z","timestamp":1791418269404,"version":"4.3.3"},"reference-count":54,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2016,5,29]],"date-time":"2016-05-29T00:00:00Z","timestamp":1464480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"R\u00e9gion des Pays de la Loire"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Reducing intestinal epithelial barrier (IEB) dysfunctions is recognized as being of major therapeutic interest for various intestinal disorders.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Sacral nerve stimulation (SNS) is known to reduce IEB permeability.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Here, we report in a pig model that SNS enhances morphological and functional recovery of IEB following mucosal injury induced via 2,4,6\u2010trinitrobenzenesulfonic acid. These effects are associated with an increased expression of tight junction proteins such as ZO\u20101 and FAK.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results establish that SNS enhances intestinal barrier repair in acute mucosal injury. They further set the scientific basis for future use of SNS as a complementary or alternative therapeutic option for the treatment of gut disorders with IEB dysfunctions such as inflammatory bowel diseases or irritable bowel syndrome.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Intestinal epithelial barrier (IEB) dysfunctions, such as increased permeability or altered healing, are central to intestinal disorders. Sacral nerve stimulation (SNS) is known to reduce IEB permeability, but its ability to modulate IEB repair remains unknown. This study aimed to characterize the impact of SNS on mucosal repair following 2,4,6\u2010trinitrobenzenesulfonic acid (TNBS)\u2010induced lesions. Six pigs were stimulated by SNS 3\u00a0h prior to and 3\u00a0h after TNBS enema, while sham animals (<jats:italic>n<\/jats:italic>\u00a0=\u00a08) were not stimulated. The impact of SNS on mucosal changes was evaluated by combining <jats:italic>in vivo<\/jats:italic> imaging, histological and functional methods. Biochemical and transcriptomic approaches were used to analyse the IEB and mucosal inflammatory response. We observed that SNS enhanced the recovery from TNBS\u2010induced increase in transcellular permeability. At 24\u00a0h, TNBS\u2010induced alterations of mucosal morphology were significantly less in SNS compared with sham animals. SNS reduced TNBS\u2010induced changes in ZO\u20101 expression and its epithelial pericellular distribution, and also increased pFAK\/FAK expression compared with sham. Interestingly, SNS increased the mucosal density of neutrophils, which was correlated with an increase in trypsin and TGF\u2010\u03b21 levels compared with sham. Finally, SNS prevented the TNBS\u2010induced increases in IL\u20101\u03b2 and IL\u20104 over time that were observed with sham treatment. In conclusion, our results show that SNS enhances mucosal repair following injury. This study highlights novel mechanisms of action of SNS and identifies SNS as a new therapy for diseases with IEB repair disorders.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp271783","type":"journal-article","created":{"date-parts":[[2016,3,5]],"date-time":"2016-03-05T17:16:33Z","timestamp":1457198193000},"page":"4309-4323","source":"Crossref","is-referenced-by-count":35,"title":["Sacral nerve stimulation enhances early intestinal mucosal repair following mucosal injury in a pig model"],"prefix":"10.1113","volume":"594","author":[{"given":"J\u00e9r\u00e9my","family":"Br\u00e9geon","sequence":"first","affiliation":[{"name":"INSERM  U913 Nantes F\u201044093 France"},{"name":"Universit\u00e9 Nantes  Nantes F\u201044093 France"},{"name":"CHU Nantes, H\u00f4tel Dieu Institut des Maladies de l'Appareil Digestif  F\u201044093 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Coron","sequence":"additional","affiliation":[{"name":"INSERM  U913 Nantes 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