{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T16:04:25Z","timestamp":1780934665696,"version":"3.54.1"},"reference-count":112,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T00:00:00Z","timestamp":1460505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Carotid body (CB) glomus cells mediate acute oxygen sensing and the initiation of the hypoxic ventilatory response, yet the gene expression profile of these cells is not available.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We demonstrate that the single cell RNA\u2010Seq method is a powerful tool for identifying highly expressed genes in CB glomus cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our single cell RNA\u2010Seq results characterized novel CB glomus cell genes, including members of the G protein\u2010coupled receptor signalling pathway, ion channels and atypical mitochondrial electron transport chain subunits.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A heterologous cell\u2010based screening identified acetate (which is known to affect CB glomus cell activity) as an agonist for the most highly abundant G protein\u2010coupled receptor (Olfr78) in CB glomus cells.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These data established the first transcriptome profile of CB glomus cells, highlighting genes with potential implications in CB chemosensory function.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood content, including oxygen. Despite significant advancements in the characterization of their physiological properties, our understanding of the underlying molecular machinery and signalling pathway in CB glomus cells is still limited. To overcome this, we employed the single cell RNA\u00adSeq method by performing next\u2010generation sequencing on single glomus cell\u00adderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. Using this method, we identified a set of genes abundantly expressed in glomus cells, which contained novel glomus cell\u2010specific genes. Transcriptome and subsequent <jats:italic>in situ<\/jats:italic> hybridization and immunohistochemistry analyses identified abundant G protein\u2010coupled receptor signalling pathway components and various types of ion channels, as well as members of the hypoxia\u2010inducible factors pathway. A short\u2010chain fatty acid olfactory receptor Olfr78, recently implicated in CB function, was the most abundant G protein\u2010coupled receptor. Two atypical mitochondrial electron transport chain subunits (Ndufa4l2 and Cox4i2) were among the most specifically expressed genes in CB glomus cells, highlighting their potential roles in mitochondria\u2010mediated oxygen sensing. The wealth of information provided by the present study offers a valuable foundation for identifying molecules functioning in the CB.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp271936","type":"journal-article","created":{"date-parts":[[2016,3,5]],"date-time":"2016-03-05T17:12:55Z","timestamp":1457197975000},"page":"4225-4251","source":"Crossref","is-referenced-by-count":107,"title":["Single cell transcriptome analysis of mouse carotid body glomus cells"],"prefix":"10.1113","volume":"594","author":[{"given":"Ting","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology Duke University Medical Centre  Durham NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010Shan","family":"Chien","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology Duke University Medical Centre  Durham NC 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