{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:12:34Z","timestamp":1784041954191,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T00:00:00Z","timestamp":1468800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>E74\u2010like factor 3 (ELF3) is a transcription factor regulated by inflammation in different physio\u2010pathological situations. Lipocalin\u20102 (LCN2) emerged as a relevant adipokine involved in the regulation of inflammation. In this study we showed for the first time the involvement of ELF3 in the control of LCN2 expression and its cooperation with nuclear factor\u2010\u03baB (NF\u03baB).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our results will help to better understand of the role of ELF3, NF\u03baB and LCN2 in the pathophysiology of articular cartilage.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>E74\u2010like factor 3 (ELF3) is a transcription factor induced by inflammatory cytokines in chondrocytes that increases gene expression of catabolic and inflammatory mediators. Lipocalin 2 (LCN2) is a novel adipokine that negatively impacts articular cartilage, triggering catabolic and inflammatory responses in chondrocytes. Here, we investigated the control of LCN2 gene expression by ELF3 in the context of interleukin 1 (IL\u20101)\u2010driven inflammatory responses in chondrocytes. The interaction of ELF3 and nuclear factor\u2010\u03baB (NF\u03baB) in modulating LCN2 levels was also explored. LCN2 mRNA and protein levels, as well those of several other ELF3 target genes, were determined by RT\u2010qPCR and Western blotting. Human primary chondrocytes, primary chondrocytes from wild\u2010type and <jats:italic>Elf3<\/jats:italic> knockout mice, and immortalized human T\/C\u201028a2 and murine ATDC5 cell lines were used in <jats:italic>in vitro<\/jats:italic> assays. The activities of various gene reporter constructs were evaluated by luciferase assays. Gene overexpression and knockdown were performed using specific expression vectors and siRNA technology, respectively. ELF3 overexpression transactivated the LCN2 promoter and increased the IL\u20101\u2010induced mRNA and protein levels of LCN2, as well as the mRNA expression of other pro\u2010inflammatory mediators, in human and mouse chondrocytes. We also identified a collaborative loop between ELF3 and NF\u03baB that amplifies the induction of LCN2. Our findings show a novel role for ELF3 and NF\u03baB in the induction of the pro\u2010inflammatory adipokine LCN2, providing additional evidence of the interaction between ELF3 and NF\u03baB in modulating inflammatory responses, and a better understanding of the mechanisms of action of ELF3 in chondrocytes.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp272240","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T08:15:27Z","timestamp":1464164127000},"page":"6133-6146","source":"Crossref","is-referenced-by-count":27,"title":["E74\u2010like factor 3 and nuclear factor\u2010\u03baB regulate lipocalin\u20102 expression in chondrocytes"],"prefix":"10.1113","volume":"594","author":[{"given":"Javier","family":"Conde","sequence":"first","affiliation":[{"name":"SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigaci\u00f3n Sanitaria de Santiago) Research Laboratory 9 The NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital  Santiago de Compostela 15706 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Otero","sequence":"additional","affiliation":[{"name":"Tissue Engineering Regeneration and Repair Program, The Hospital for Special Surgery, and Department of Cell and Developmental Biology Weill Cornell Medical College  New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morena","family":"Scotece","sequence":"additional","affiliation":[{"name":"SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigaci\u00f3n Sanitaria de Santiago) Research Laboratory 9 The NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital  Santiago de Compostela 15706 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Abella","sequence":"additional","affiliation":[{"name":"SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigaci\u00f3n Sanitaria de Santiago) Research Laboratory 9 The NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital  Santiago de Compostela 15706 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigaci\u00f3n Sanitaria de Santiago) Research Laboratory 9 The NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital  Santiago de Compostela 15706 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Pino","sequence":"additional","affiliation":[{"name":"SERGAS (Servizo Gallego de Saude) Santiago University Clinical Hospital Division of Orthopaedic Surgery  Santiago de Compostela Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigaci\u00f3n Sanitaria de Santiago) Research Laboratory 9 The NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital  Santiago de Compostela 15706 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Lago","sequence":"additional","affiliation":[{"name":"SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigaci\u00f3n Sanitaria de Santiago) Research Laboratory 9 The NEIRID Lab (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital  Santiago de Compostela 15706 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary B.","family":"Goldring","sequence":"additional","affiliation":[{"name":"Tissue 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