{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:14:03Z","timestamp":1765484043986},"reference-count":86,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T00:00:00Z","timestamp":1473897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Earth and Life Sciences-Netherlands Organisation of Scientific Research","award":["#823.02.006"],"award-info":[{"award-number":["#823.02.006"]}]},{"name":"Dutch Fund for Economic Structure Reinforcement","award":["FES, 0908"],"award-info":[{"award-number":["FES, 0908"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Neurons in the medial nucleus of the trapezoid body of anaesthetized rats of postnatal day (P)2\u20136 showed burst firing with a preferred interval of about 100\u00a0ms, which was stable, and a second preferred interval of 5\u201330\u00a0ms, which shortened during development.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In 3 out of 132 cases, evidence for the presence of two large inputs was found.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:italic>In vivo<\/jats:italic> whole\u2010cell recordings revealed that the excitability of the principal neuron and the size of its largest synaptic inputs were developmentally matched.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>At P2\u20134, action potentials were triggered by barrages of small synaptic events that summated to plateau potentials, while at later stages firing depended on a single, large and often prespike\u2010associated input, which is probably the nascent calyx of Held.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Simulations with a Hodgkin\u2013Huxley\u2010like model, which was based on fits of the intrinsic postsynaptic properties, suggested an essential role for the low\u2010threshold potassium conductance in this transition.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>In the adult, principal neurons of the medial nucleus of the trapezoid body (MNTB) are typically contacted by a single, giant terminal called the calyx of Held, whereas during early development a principal neuron receives inputs from many axons. How these changes in innervation impact the postsynaptic activity has not yet been studied <jats:italic>in vivo<\/jats:italic>. We therefore recorded spontaneous inputs and intrinsic properties of principal neurons in anaesthetized rat pups during the developmental period in which the calyx forms. A characteristic bursting pattern could already be observed at postnatal day (P)2, before formation of the calyx. At this age, action potentials (APs) were triggered by barrages of summating EPSPs causing plateau depolarizations. In contrast, at P5, a single EPSP reliably triggered APs, resulting in a close match between pre\u2010 and postsynaptic firing. Postsynaptic excitability and the size of the largest synaptic events were developmentally matched. The developmental changes in intrinsic properties were estimated by fitting <jats:italic>in vivo<\/jats:italic> current injections to a Hodgkin\u2013Huxley\u2010type model of the principal neuron. Our simulations indicated that the developmental increases in <jats:italic>I<\/jats:italic><jats:sub>h<\/jats:sub>, low\u2010threshold K<jats:sup>+<\/jats:sup> channels and leak currents contributed to the reduction in postsynaptic excitability, but that low\u2010threshold K<jats:sup>+<\/jats:sup> channels specifically functioned as a dampening influence in the near\u2010threshold range, thus precluding small inputs from triggering APs. Together, these coincident changes help to propagate bursting activity along the auditory brainstem, and are essential steps towards establishing the relay function of the calyx of Held synapse.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp272780","type":"journal-article","created":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T10:03:00Z","timestamp":1468836180000},"page":"207-231","source":"Crossref","is-referenced-by-count":11,"title":["<i>In vivo<\/i> matching of postsynaptic excitability with spontaneous synaptic inputs during formation of the rat calyx of Held synapse"],"prefix":"10.1113","volume":"595","author":[{"given":"Martijn C.","family":"Sierksma","sequence":"first","affiliation":[{"name":"Department of Neuroscience Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milly S.","family":"Tedja","sequence":"additional","affiliation":[{"name":"Department of Neuroscience Erasmus MC University Medical Center Rotterdam  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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