{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:56:04Z","timestamp":1784001364310,"version":"3.55.0"},"reference-count":75,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2017,7,5]],"date-time":"2017-07-05T00:00:00Z","timestamp":1499212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG034389"],"award-info":[{"award-number":["R01AG034389"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS082759"],"award-info":[{"award-number":["R01NS082759"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS095215"],"award-info":[{"award-number":["R01NS095215"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>At rat calyx of Held terminals, ATP was required not only for slow endocytosis, but also for rapid phase of compensatory endocytosis.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>An ATP\u2010independent form of endocytosis was recruited to accelerate membrane retrieval at increased activity and temperature.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ATP\u2010independent endocytosis primarily involved retrieval of pre\u2010existing membrane, which depended on Ca<jats:sup>2+<\/jats:sup> and the activity of neutral sphingomyelinase but not clathrin\u2010coated pit maturation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>ATP\u2010independent endocytosis represents a non\u2010canonical mechanism that can efficiently retrieve membrane at physiological conditions without competing for the limited ATP at elevated neuronal activity.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Neurotransmission relies on membrane endocytosis to maintain vesicle supply and membrane stability. Endocytosis has been generally recognized as a major ATP\u2010dependent function, which efficiently retrieves more membrane at elevated neuronal activity when ATP consumption within nerve terminals increases drastically. This paradox raises the interesting question of whether increased activity recruits ATP\u2010independent mechanism(s) to accelerate endocytosis at the same time as preserving ATP availability for other tasks. To address this issue, we studied ATP requirement in three typical forms of endocytosis at rat calyx of Held terminals by whole\u2010cell membrane capacitance measurements. At room temperature, blocking ATP hydrolysis effectively abolished slow endocytosis and rapid endocytosis but only partially inhibited excess endocytosis following intense stimulation. The ATP\u2010independent endocytosis occurred at calyces from postnatal days 8\u201315, suggesting its existence before and after hearing onset. This endocytosis was not affected by a reduction of exocytosis using the light chain of botulinum toxin C, nor by block of clathrin\u2010coat maturation. It was abolished by EGTA, which preferentially blocked endocytosis of retrievable membrane pre\u2010existing at the surface, and was impaired by oxidation of cholesterol and inhibition of neutral sphingomyelinase. ATP\u2010independent endocytosis became more significant at 34\u201335\u00b0C, and recovered membrane by an amount that, on average, was close to exocytosis. The results of the present study suggest that activity and temperature recruit ATP\u2010independent endocytosis of pre\u2010existing membrane (in addition to ATP\u2010dependent endocytosis) to efficiently retrieve membrane at nerve terminals. This less understood endocytosis represents a non\u2010canonical mechanism regulated by lipids such as cholesterol and sphingomyelinase.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp274275","type":"journal-article","created":{"date-parts":[[2017,5,29]],"date-time":"2017-05-29T06:22:41Z","timestamp":1496038961000},"page":"5265-5284","source":"Crossref","is-referenced-by-count":13,"title":["Promotion of endocytosis efficiency through an ATP\u2010independent mechanism at rat calyx of Held terminals"],"prefix":"10.1113","volume":"595","author":[{"given":"Hai\u2010Yuan","family":"Yue","sequence":"first","affiliation":[{"name":"Departments of Neuroscience and Regenerative Medicine Augusta University  USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erhard","family":"Bieberich","sequence":"additional","affiliation":[{"name":"Departments of Neuroscience and Regenerative Medicine Augusta University  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