{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:45Z","timestamp":1787016225976,"version":"build-2736575974"},"reference-count":67,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T00:00:00Z","timestamp":1527033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1140468"],"award-info":[{"award-number":["1140468"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002590","name":"American Lung Association","doi-asserted-by":"publisher","award":["RG\u2010196192\u2010N"],"award-info":[{"award-number":["RG\u2010196192\u2010N"]}],"id":[{"id":"10.13039\/100002590","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["11SDG5670050"],"award-info":[{"award-number":["11SDG5670050"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>We investigated the excitation\u2013contraction coupling mechanisms in small pulmonary veins (SPVs) in rat precision\u2010cut lung slices.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>We found that SPVs contract strongly and reversibly in response to extracellular ATP and other vasoconstrictors, including angiotensin\u2010II and endothelin\u20101.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            ATP\u2010induced vasoconstriction in SPVs was associated with the stimulation of purinergic P2Y2 receptors in vascular smooth muscle cell, activation of phospholipase C\u2010\u03b2 and the generation of intracellular Ca\n                            <jats:sup>2+<\/jats:sup>\n                            oscillations mediated by cyclic Ca\n                            <jats:sup>2+<\/jats:sup>\n                            release events via the inositol 1,4,5\u2010trisphosphate receptor.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Active constriction of SPVs may play an important role in the development of pulmonary hypertension and pulmonary oedema.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      The small pulmonary veins (SPVs) may play a role in the development of pulmonary hypertension and pulmonary oedema via active changes in SPV diameter, mediated by vascular smooth muscle cell (VSMC) contraction. However, the excitation\u2013contraction coupling mechanisms during vasoconstrictor stimulation remain poorly understood in these veins. We used rat precision\u2010cut lung slices and phase\u2010contrast and confocal microscopy to investigate dynamic changes in SPV cross\u2010sectional luminal area and intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      signalling in their VSMCs. We found that the SPV (\u223c150\u00a0\u03bcm in diameter) contract strongly in response to extracellular ATP and other vasoconstrictors, including angiotensin\u2010II and endothelin\u20101. ATP\u2010induced SPV contraction was fast, concentration\u2010dependent, completely reversible upon ATP washout, and inhibited by purinergic receptor antagonists suramin and AR\u2010C118925 but not by MRS2179. Immunofluorescence showed purinergic P2Y2 receptors expressed in SPV VSMCs. ATP\u2010induced SPV contraction was inhibited by phospholipase C\u03b2 inhibitor U73122 and accompanied by intracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      oscillations in the VSMCs. These Ca\n                      <jats:sup>2+<\/jats:sup>\n                      oscillations and SPV contraction were inhibited by the inositol 1,4,5\u2010trisphosphate receptor inhibitor 2\u2010APB but not by ryanodine. The results of the present study suggest that ATP\u2010induced vasoconstriction in SPVs is associated with the activation of purinergic P2Y2 receptors in VSMCs and the generation of Ca\n                      <jats:sup>2+<\/jats:sup>\n                      oscillations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp274731","type":"journal-article","created":{"date-parts":[[2018,5,23]],"date-time":"2018-05-23T03:55:59Z","timestamp":1527047759000},"page":"2491-2506","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Purinergic receptor stimulation induces calcium oscillations and smooth muscle contraction in small pulmonary veins"],"prefix":"10.1113","volume":"596","author":[{"given":"Mauricio","family":"Henriquez","sequence":"first","affiliation":[{"name":"Program of Physiology and Biophysics, ICBM, Faculty of Medicine University of Chile Independencia 1027 Santiago 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