{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T02:28:47Z","timestamp":1781836127854,"version":"3.54.5"},"reference-count":91,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2018,10,13]],"date-time":"2018-10-13T00:00:00Z","timestamp":1539388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK078736"],"award-info":[{"award-number":["DK078736"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Inhibitory neuromuscular transmission (NMT) was compared in the internal anal sphincter (IAS) and rectum of the <jats:italic>Cynomolgus<\/jats:italic> monkey, an animal with high gene sequence identity to humans.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Nitrergic NMT was present in both muscles while purinergic NMT was limited to the rectum and VIPergic NMT to the IAS. The profile for monkey IAS more closely resembles humans than rodents.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In both muscles, SK3 channels were localized to PDGFR\u03b1<jats:sup>+<\/jats:sup> cells that were closely associated with nNOS<jats:sup>+<\/jats:sup>\/VIP<jats:sup>+<\/jats:sup> nerves. Gene expression levels of P2RY subtypes were the same in IAS and rectum while KCNN expression levels were very similar.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>SK3 channel activation and inhibition caused faster\/greater changes in contractile activity in rectum than IAS. P2Y1 receptor activation inhibited contraction in rectum while increasing contraction in IAS.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The absence of purinergic NMT in the IAS may be due to poor coupling between P2Y1 receptors and SK3 channels on PDGFR\u03b1<jats:sup>+<\/jats:sup> cells.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Inhibitory neuromuscular transmission (NMT) was compared in the internal anal sphincter (IAS) and rectum of the <jats:italic>Cynomolgus<\/jats:italic> monkey, an animal with a high gene sequence identity to humans. Electrical field stimulation produced nitric oxide synthase (NOS)\u2010dependent contractile inhibition in both muscles whereas P2Y1\u2010dependent purinergic NMT was restricted to rectum. An additional NOS\u2010independent, \u03b1\u2010chymotrypsin\u2010sensitive component was identified in the IAS consistent with vasoactive intestinal peptide\u2010ergic (VIPergic) NMT. Microelectrode recordings revealed slow NOS\u2010dependent inhibitory junction potentials (IJPs) in both muscles and fast P2Y1\u2010dependent IJPs in rectum. The basis for the difference in purinergic NMT was investigated. PDGFR\u03b1<jats:sup>+<\/jats:sup>\/SK3<jats:sup>+<\/jats:sup> cells were closely aligned with nNOS<jats:sup>+<\/jats:sup>\/VIP<jats:sup>+<\/jats:sup> neurons in both muscles. Gene expression of P2RY was the same in IAS and rectum (P2RY1&gt;&gt;P2RY2\u201014) while KCNN3 expression was 32% greater in rectum. The SK channel inhibitor apamin doubled contractile activity in rectum while having minimal effect in the IAS. Contractile inhibition elicited with the SK channel agonist CyPPA was five times faster in rectum than in the IAS. The P2Y1 receptor agonist MRS2365 inhibited contraction in rectum but increased contraction in the IAS. In conclusion, both the IAS and the rectum have nitrergic NMT whereas purinergic NMT is limited to rectum and VIPergic NMT to the IAS. The profile in monkey IAS more closely resembles that of humans than rodents. The lack of purinergic NMT in the IAS cannot be attributed to the absence of PDGFR\u03b1<jats:sup>+<\/jats:sup> cells, P2Y1 receptors or SK3 channels. Rather, it appears to be due to poor coupling between P2Y1 receptors and SK3 channels on PDGFR\u03b1<jats:sup>+<\/jats:sup> cells.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp275437","type":"journal-article","created":{"date-parts":[[2018,9,10]],"date-time":"2018-09-10T06:58:13Z","timestamp":1536562693000},"page":"5319-5341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Comparison of inhibitory neuromuscular transmission in the <i>Cynomolgus<\/i> monkey IAS and rectum: special emphasis on differences in purinergic transmission"],"prefix":"10.1113","volume":"596","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2871-3825","authenticated-orcid":false,"given":"C. 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