{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:40:52Z","timestamp":1780414852585,"version":"3.54.1"},"reference-count":41,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T00:00:00Z","timestamp":1555459200000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T00:00:00Z","timestamp":1523923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006130","name":"Office of Electricity Delivery and Energy Reliability","doi-asserted-by":"publisher","award":["284438"],"award-info":[{"award-number":["284438"]}],"id":[{"id":"10.13039\/100006130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006130","name":"Office of Electricity Delivery and Energy Reliability","doi-asserted-by":"publisher","award":["40001043721\/11\/NL\/KML"],"award-info":[{"award-number":["40001043721\/11\/NL\/KML"]}],"id":[{"id":"10.13039\/100006130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Key points<\/jats:title>\nSuperposition of hypoxia on 21\u00a0day bed rest did not worsen the impairment of skeletal muscle oxidative function induced by bed rest alone.<\/jats:p><\/jats:list-item>\nA significant impairment of maximal oxidative performance was identified downstream of cardiovascular O2<\/jats:sub> delivery, involving both the intramuscular matching between O2<\/jats:sub> supply and utilization and mitochondrial respiration.<\/jats:p><\/jats:list-item>\nThese chronic adaptations appear to be relevant in terms of exposure to spaceflights and reduced gravity habitats (Moon or Mars), as characterized by low gravity and hypoxia, in patients with chronic diseases characterized by hypomobility\/immobility and hypoxia, as well as in ageing.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>Abstract<\/jats:title>Skeletal muscle oxidative function was evaluated in 11 healthy males (mean\u00a0\u00b1\u00a0SD age 27\u00a0\u00b1\u00a05\u00a0years) prior to (baseline data collection, BDC) and following a 21\u00a0day horizontal bed rest (BR), carried out in normoxia (\u00a0=\u00a0133\u00a0mmHg; N\u2010BR) and hypoxia (\u00a0=\u00a090\u00a0mmHg; H\u2010BR). H\u2010BR was aimed at simulating reduced gravity habitats. The effects of a 21\u00a0day hypoxic ambulatory confinement (\u00a0=\u00a090\u00a0mmHg; H\u2010AMB) were also assessed. Pulmonary O2<\/jats:sub> uptake (), vastus lateralis fractional O2<\/jats:sub> extraction (changes in deoxygenated haemoglobin\u00a0+\u00a0myoglobin concentration, \u0394[deoxy(Hb\u00a0+\u00a0Mb)]; near\u2010infrared spectroscopy) and femoral artery blood flow (ultrasound Doppler) were evaluated during incremental one\u2010leg knee\u2010extension exercise (reduced constraints to cardiovascular O2<\/jats:sub> delivery) carried out to voluntary exhaustion in a normoxic environment. Mitochondrial respiration was evaluated ex vivo<\/jats:italic> by high\u2010resolution respirometry in permeabilized vastus lateralis fibres. decreased (P<\/jats:italic>\u00a0<\u00a00.05) after N\u2010BR (0.98\u00a0\u00b1\u00a00.13\u00a0L\u00a0min\u22121<\/jats:sup>) and H\u2010BR (0.96\u00a0\u00b1\u00a00.17\u00a0L\u00a0min\u22121<\/jats:sup>) vs<\/jats:italic>. BDC (1.05\u00a0\u00b1\u00a00.14\u00a0L min\u22121<\/jats:sup>). In the presence of a decreased (by \u223c6\u20138%) thigh muscle volume, normalized per unit of muscle mass was not affected by both interventions. \u0394[deoxy(Hb\u00a0+\u00a0Mb)]peak<\/jats:sub> decreased (P<\/jats:italic>\u00a0<\u00a00.05) after N\u2010BR (65\u00a0\u00b1\u00a013% of limb ischaemia) and H\u2010BR (62\u00a0\u00b1\u00a012%) vs<\/jats:italic>. BDC (73\u00a0\u00b1\u00a013%). H\u2010AMB did not alter or \u0394[deoxy(Hb\u00a0+\u00a0Mb)]peak<\/jats:sub>. An overshoot of \u0394[deoxy(Hb\u00a0+\u00a0Mb)] was evident during the first minute of unloaded exercise after N\u2010BR and H\u2010BR. Arterial blood flow to the lower limb during both unloaded and peak knee extension was not affected by any intervention. Maximal ADP\u2010stimulated mitochondrial respiration decreased (P<\/jats:italic>\u00a0<\u00a00.05) after all interventions vs<\/jats:italic>. control. In 21\u00a0day N\u2010BR, a significant impairment of oxidative metabolism occurred downstream of cardiovascular O2<\/jats:sub> delivery, affecting both mitochondrial respiration and presumably the intramuscular matching between O2<\/jats:sub> supply and utilization. Superposition of H on BR did not worsen the impairment induced by BR alone.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp275605","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T20:21:33Z","timestamp":1523996493000},"page":"3341-3355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["PlanHab*<\/sup>: hypoxia does not worsen the impairment of skeletal muscle oxidative function induced by bed rest alone"],"prefix":"10.1113","volume":"596","author":[{"given":"Desy","family":"Salvadego","sequence":"first","affiliation":[{"name":"Department of Medicine University of Udine Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7440-2171","authenticated-orcid":false,"given":"Michail E.","family":"Keramidas","sequence":"additional","affiliation":[{"name":"Department of Environmental Physiology, Swedish Aerospace Physiology Centre Royal Institute of Technology Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"K\u00f6leg\u00e5rd","sequence":"additional","affiliation":[{"name":"Department of Environmental Physiology, Swedish Aerospace Physiology Centre Royal Institute of Technology Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1048-0573","authenticated-orcid":false,"given":"Lorenza","family":"Brocca","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Pavia Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Lazzer","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Mavelli","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine Udine 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