{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T11:21:22Z","timestamp":1777634482781,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,4,15]],"date-time":"2018-04-15T00:00:00Z","timestamp":1523750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL122228"],"award-info":[{"award-number":["HL122228"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK116311"],"award-info":[{"award-number":["DK116311"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2018,4,15]]},"abstract":"\n Key points<\/jats:title>\n \n \n \n \n The action potential initiation in the nerve terminals and its subsequent conduction along the axons of afferent nerves are not necessarily dependent on the same voltage\u2010gated sodium channel (Na\n V<\/jats:sub>\n 1) subunits.\n <\/jats:p>\n <\/jats:list-item>\n \n The action potential initiation in jugular C\u2010fibres within airway tissues is not blocked by TTX; nonetheless, conduction of action potentials along the vagal axons of these nerves is often dependent on TTX\u2010sensitive channels.<\/jats:p>\n <\/jats:list-item>\n \n \n This is not the case for nodose airway A\u03b4\u2010fibres and C\u2010fibres, where both action potential initiation and conduction is abolished by TTX or selective Na\n V<\/jats:sub>\n 1.7 blockers.\n <\/jats:p>\n <\/jats:list-item>\n \n \n The difference between the initiation of action potentials within the airways\n vs<\/jats:italic>\n . conduction along the axons should be considered when developing Na\n V<\/jats:sub>\n 1 blocking drugs for topical application to the respiratory tract.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Abstract<\/jats:title>\n \n The action potential (AP) initiation in the nerve terminals and its subsequent AP conduction along the axons do not necessarily depend on the same subtypes of voltage\u2010gated sodium channels (Na\n V<\/jats:sub>\n 1s). We evaluated the role of TTX\u2010sensitive and TTX\u2010resistant Na\n V<\/jats:sub>\n 1s in vagal afferent nociceptor nerves derived from jugular and nodose ganglia innervating the respiratory system. Single cell RT\u2010PCR was performed on vagal afferent neurons retrogradely labelled from the guinea pig trachea. Almost all of the jugular neurons expressed the TTX\u2010sensitive channel Na\n V<\/jats:sub>\n 1.7 along with TTX\u2010resistant Na\n V<\/jats:sub>\n 1.8 and Na\n V<\/jats:sub>\n 1.9. Tracheal nodose neurons also expressed Na\n V<\/jats:sub>\n 1.7 but, less frequently, Na\n V<\/jats:sub>\n 1.8 and Na\n V<\/jats:sub>\n 1.9. Na\n V<\/jats:sub>\n 1.6 were expressed in \u223c40% of the jugular and 25% of nodose tracheal neurons. Other Na\n V<\/jats:sub>\n 1 \u03b1 subunits were only rarely expressed. Single fibre recordings were made from the vagal nodose and jugular nerve fibres innervating the trachea or lung in the isolated perfused vagally\u2010innervated preparations that allowed for selective drug delivery to the nerve terminal compartment (AP initiation) or to the desheathed vagus nerve (AP conduction). AP initiation in jugular C\u2010fibres was unaffected by TTX, although it was inhibited by Na\n V<\/jats:sub>\n 1.8 blocker (PF\u201001247324) and abolished by combination of TTX and PF\u201001247324. However, AP conduction in the majority of jugular C\u2010fibres was abolished by TTX. By contrast, both AP initiation and conduction in nodose nociceptors was abolished by TTX or selective Na\n V<\/jats:sub>\n 1.7 blockers. Distinction between the effect of a drug with respect to inhibiting AP in the nerve terminals within the airways\n vs<\/jats:italic>\n . at conduction sites along the vagus nerve is relevant to therapeutic strategies involving inhaled Na\n V<\/jats:sub>\n 1 blocking drugs.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp275698","type":"journal-article","created":{"date-parts":[[2018,2,12]],"date-time":"2018-02-12T22:52:50Z","timestamp":1518475970000},"page":"1419-1432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Different role of TTX\u2010sensitive voltage\u2010gated sodium channel (Na\n V<\/sub>\n 1) subtypes in action potential initiation and conduction in vagal airway nociceptors"],"prefix":"10.1113","volume":"596","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9659-5007","authenticated-orcid":false,"given":"M.","family":"Kollarik","sequence":"first","affiliation":[{"name":"Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD USA"},{"name":"Department of Pathophysiology, Biomedical Center Martin, Jessenius Faculty of Medicine in Martin Comenius University in Bratislava Martin Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2303-0144","authenticated-orcid":false,"given":"R. A.","family":"Herbstsomer","sequence":"additional","affiliation":[{"name":"Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ru","sequence":"additional","affiliation":[{"name":"Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9510-7764","authenticated-orcid":false,"given":"M.","family":"Kocmalova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Biomedical Center Martin, Jessenius Faculty of Medicine in Martin Comenius University in Bratislava Martin Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. N.","family":"Meeker","sequence":"additional","affiliation":[{"name":"Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5299-9029","authenticated-orcid":false,"given":"B. J.","family":"Undem","sequence":"additional","affiliation":[{"name":"Department of Medicine The Johns Hopkins University School of Medicine Baltimore MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152405"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0156"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.211bf.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.057885"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.224204"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-41588-3_6"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.01.015"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP270818"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1952.0054"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.159.6.9808078"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1016\/S0022-3565(24)38188-1","article-title":"Characterization of vagal afferent subtypes stimulated by bradykinin in guinea pig trachea","volume":"289","author":"Kajekar R","year":"1999","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.116.238469"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.022848"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.146365"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c130020"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4354-08.2009"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2015"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00028.2013"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.215384"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13092"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021703"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021234"},{"key":"e_1_2_5_24_1","first-page":"365","volume-title":"Bioinformatics Methods and Protocols: Methods in Molecular Biology","author":"Rozen S","year":"2000"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autneu.2012.10.014"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.060079"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-20-07909.2001"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185985"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2006.04.073"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2004.12.002"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2FJP275698","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP275698","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T15:08:49Z","timestamp":1751382529000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP275698"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,15]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,4,15]]}},"alternative-id":["10.1113\/JP275698"],"URL":"https:\/\/doi.org\/10.1113\/jp275698","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.732647795.793542691","asserted-by":"object"}]},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,15]]},"assertion":[{"value":"2017-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}