{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:44:06Z","timestamp":1790289846197,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T00:00:00Z","timestamp":1545782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"AHA Scientist Development Grant","award":["12SDG12050697"],"award-info":[{"award-number":["12SDG12050697"]}]},{"name":"AHA Beginning Grant-in-Aid","award":["11BGIA7710059"],"award-info":[{"award-number":["11BGIA7710059"]}]},{"name":"NIH Center of Biomedical Research Excellence on Obesity and Cardiovascular Diseases","award":["P20 GM103527-06"],"award-info":[{"award-number":["P20 GM103527-06"]}]},{"name":"Startup fund from Wright State University"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Lipin1 is critical for skeletal muscle development.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Lipin1 regulates MyoD and myocyte\u2010specific enhancer factor 2C (MEF2c) expression via the protein kinase C (PKC)\/histone deacetylase 5\u2010mediated pathway.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Inhibition of PKC\u03bc activity suppresses myoblast differentiation by inhibiting MyoD and MEF2c expression.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Our previous characterization of global lipin1\u2010deficient (<jats:italic>fld<\/jats:italic>) mice demonstrated that lipin1 played a novel role in skeletal muscle (SM) regeneration. The present study using cell type\u2010specific Myf5\u2010cre;Lipin1<jats:sup>fl\/fl<\/jats:sup> conditional knockout mice (Lipin1<jats:sup>Myf5cKO<\/jats:sup>) shows that lipin1 is a major determinant of SM development. Lipin1 deficiency induced reduced muscle mass and myopathy. Our results from lipin1\u2010deficient myoblasts suggested that lipin1 regulates myoblast differentiation via the protein kinase C\u03bc (PKC\u03bc)\/histone deacetylase 5 (HDAC5)\/myocyte\u2010specific enhancer factor 2C (MEF2c):MyoD\u2010mediated pathway. Lipin1 deficiency leads to the suppression of PKC isoform activities, as well as inhibition of the downstream target of PKC\u03bc, class II deacetylase HDAC5 nuclear export, and, consequently, inhibition of MEF2c and MyoD expression in the SM of lipin1<jats:sup>Myf5cKO<\/jats:sup> mice. Restoration of diacylglycerol\u2010mediated signalling in lipin1 deficient myoblasts by phorbol 12\u2010myristate 13\u2010acetate transiently activated PKC and HDAC5, and upregulated MEF2c expression. Our findings provide insights into the signalling circuitry that regulates SM development, and have important implications for developing intervention aimed at treating muscular dystrophy.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp276919","type":"journal-article","created":{"date-parts":[[2018,12,4]],"date-time":"2018-12-04T12:49:57Z","timestamp":1543927797000},"page":"889-901","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Lipin1 is required for skeletal muscle development by regulating MEF2c and MyoD expression"],"prefix":"10.1113","volume":"597","author":[{"given":"Abdulrahman","family":"Jama","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Wright State University  Dayton OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengtong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology 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