{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:13:12Z","timestamp":1792624392220,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,3]],"date-time":"2019-02-03T00:00:00Z","timestamp":1549152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["17PRE33410260"],"award-info":[{"award-number":["17PRE33410260"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AR067450"],"award-info":[{"award-number":["R01AR067450"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R37HL041026"],"award-info":[{"award-number":["R37HL041026"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Skeletal muscle regenerates after injury, however the recovery of its microvascular supply is poorly understood. We injured the gluteus maximus muscle in mice aiming to investigate the recovery of blood flow regulation in microvascular resistance networks. We hypothesized that blood flow regulation recovers in concert with myofibre regeneration.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Microvascular perfusion ceased within 1\u00a0day post injury and was restored at 5\u00a0days coincident with the appearance of new myofibres; however, the resistance network was dilated and unresponsive to vasoactive agents. Spontaneous vasomotor tone, endothelium\u2010dependent dilatation and adrenergic vasoconstriction increased at 10\u00a0days in concert with myofibre regeneration. Vasomotor control recovered at 21\u00a0days, when regenerated myofibres matured and active force production stabilized. Functional vasodilatation in response to muscle contraction recovered at 35\u00a0days.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Physiological integrity of microvascular smooth muscle and endothelium recovers in parallel with myofibre regeneration. Additional time is required to restore the efficacy of signalling between myofibres and microvascular networks controlling their oxygen supply.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Myofibre regeneration after skeletal muscle injury is well\u2010studied, although little is known about how microvascular perfusion is restored. The present study aimed to evaluate the recovery of blood flow regulation during skeletal muscle regeneration. In anaesthetized male C57BL\/6J mice (aged 4 months), the gluteus maximus muscle (GM) was injured by local injection of barium chloride solution (1.2%, 75\u00a0\u03bcL). Functional integrity of the resistance network was evaluated at 5, 10, 21 and 35 days post\u2010injury <jats:italic>vs<\/jats:italic>. Control by measuring internal diameter of feed arteries, first\u2010, second\u2010 and third\u2010order arterioles supplying the GM using intravital microscopy. The resting diameters of all branch orders were significantly greater (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) than Control at 5 and 10\u00a0days and recovered to Control by 21\u00a0days, as did spontaneous vasomotor tone. Vasodilatation to ACh and vasoconstriction to phenylephrine (10<jats:sup>\u22129<\/jats:sup> to 10<jats:sup>\u22125<\/jats:sup>\u00a0<jats:sc>m<\/jats:sc>) were absent at 5\u00a0days, increased at 10\u00a0days and recovered to Control by 21\u00a0days; reactivity improved in a distal\u2010to\u2010proximal gradient. Across branch orders, functional vasodilatation to single tetanic contraction (100\u00a0Hz, 500\u00a0ms) and to rhythmic twitch contractions (4\u00a0Hz, 30\u00a0s) was impaired at 5\u00a0days, improved through 21\u00a0days and was not different from Control at 35\u00a0days. Peak force development (g) was 60% of Control at 10\u00a0days and recovered by 21\u00a0days. Diminished vasomotor tone during the initial stages of regeneration promotes tissue perfusion as myofibre recovery begins. Recovery of tone and vasomotor responses to agonists occur in concert with myofibre regeneration. Delayed recovery of functional vasodilatation indicates that additional time is required to restore signalling between contracting myofibres and their vascular supply.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp277247","type":"journal-article","created":{"date-parts":[[2018,12,21]],"date-time":"2018-12-21T22:02:43Z","timestamp":1545429763000},"page":"1401-1417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Recovery of blood flow regulation in microvascular resistance networks during regeneration of mouse gluteus maximus muscle"],"prefix":"10.1113","volume":"597","author":[{"given":"Charmain A.","family":"Fernando","sequence":"first","affiliation":[{"name":"Department of Medical Pharmacology and Physiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron M.","family":"Pangan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Biological and Chemical Engineering"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DDW","family":"Cornelison","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences"},{"name":"Christopher S. 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