{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T02:41:09Z","timestamp":1783132869320,"version":"3.54.6"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T00:00:00Z","timestamp":1550707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004836","name":"Det Frie Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["8020\u201000084A"],"award-info":[{"award-number":["8020\u201000084A"]}],"id":[{"id":"10.13039\/501100004836","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007405","name":"Hjerteforeningen","doi-asserted-by":"publisher","award":["A6753"],"award-info":[{"award-number":["A6753"]}],"id":[{"id":"10.13039\/100007405","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004191","name":"Novo Nordisk","doi-asserted-by":"publisher","award":["NNF18OC0052021"],"award-info":[{"award-number":["NNF18OC0052021"]}],"id":[{"id":"10.13039\/501100004191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>The prevailing dogma about neurogenic regulation of vascular tone consists of major vasodilatation caused by CGRP (and possibly substance P) released from sensory\u2010motor nerves and vasoconstriction caused by noradrenaline, ATP and neuropeptode Y release from sympathetic nerves.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Most studies on perivascular nerve\u2010mediated vasodilatation are made <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the present study, we provide evidence indicating that <jats:italic>in vivo<\/jats:italic> electrical perivascular nerve stimulation in rat mesenteric small arteries causes a large \u03b21\u2010adrenoceptor\u2010mediated vasodilatation, which contrasts with a smaller vasodilatation caused by endogenous CGRP that is only visible after inhibition of Y1 NPY receptors.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Mesenteric arteries are densely innervated and the nerves are important regulators of vascular tone and hence blood pressure and blood flow. Perivascular sensory\u2010motor nerves have been shown to cause vasodilatation <jats:italic>in vitro<\/jats:italic>. However, less is known about their function <jats:italic>in vivo<\/jats:italic>. Male Wistar rats (10\u201312 weeks old; <jats:italic>n<\/jats:italic>\u00a0=\u00a072) were anaesthetized with ketamine (3\u00a0mg kg<jats:sup>\u22121<\/jats:sup>) and xylazine (0.75\u00a0mg kg<jats:sup>\u22121<\/jats:sup>) or pentobarbital (60\u00a0mg kg<jats:sup>\u22121<\/jats:sup>). After a laparotomy, a section of second\u2010order mesenteric artery was visualized in an organ bath after minimal removal of perivascular adipose tissue. The effects of electrical field stimulation (EFS) and drugs on artery diameter and blood flow were recorded with intravital microscopy and laser speckle imaging. EFS caused vasodilatation in arteries constricted with 1\u00a0\u03bc<jats:sc>m<\/jats:sc> U46619 in the presence of 140\u00a0\u03bc<jats:sc>m<\/jats:sc> suramin and 1\u00a0\u03bc<jats:sc>m<\/jats:sc> prazosin. The vasodilatation was inhibited by 1\u00a0\u03bc<jats:sc>m<\/jats:sc> tetrodotoxin and 5\u00a0\u03bc<jats:sc>m<\/jats:sc> guanethidine, although not by the 1\u00a0\u03bc<jats:sc>m<\/jats:sc> of the CGRP receptor antagonist BIBN4096bs. In the presence of 0.3\u00a0\u03bc<jats:sc>m<\/jats:sc> Y1 receptor antagonist BIBP3226, BIBN4096bs partly inhibited the vasodilatation. Atenolol at a concentration 1\u00a0\u03bc<jats:sc>m<\/jats:sc> inhibited the vasodilatation, whereas 0.1\u00a0\u03bc<jats:sc>m<\/jats:sc> of the \u03b2<jats:sub>2<\/jats:sub>\u2010adrenoceptor selective antagonist ICI\u2010118,551 had no effect. Increasing the extracellular [K<jats:sup>+<\/jats:sup>] to 20\u00a0m<jats:sc>m<\/jats:sc> caused vasodilatation but was converted to vasoconstriction in the presence of 1\u00a0\u03bc<jats:sc>m<\/jats:sc> BIBN4096bs, and constriction to 30\u00a0m<jats:sc>m<\/jats:sc> potassium was potentiated by BIBN4096bs. Atenolol but not BIBN4096bs increased contraction to EFS in the absence of suramin and prazosin. In mesenteric small arteries of anaesthetized rats, EFS failed to stimulate major dilatation via sensory\u2010motor nerves but induced sympathetic \u03b2<jats:sub>1<\/jats:sub>\u2010adrenoceptor\u2010mediated dilatation.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp277368","type":"journal-article","created":{"date-parts":[[2019,1,29]],"date-time":"2019-01-29T10:02:58Z","timestamp":1548756178000},"page":"1819-1831","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Rat mesenteric small artery neurogenic dilatation is predominantly mediated by \u03b2<sub>1<\/sub>\u2010adrenoceptors <i>in vivo<\/i>"],"prefix":"10.1113","volume":"597","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9608-3300","authenticated-orcid":false,"given":"Asger Maare","family":"S\u00f8ndergaard","sequence":"first","affiliation":[{"name":"Department of Biomedicine Membranes University of Aarhus  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathrine Bang","family":"Overgaard","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Membranes University of Aarhus  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Mazur","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Membranes University of Aarhus  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9708-8453","authenticated-orcid":false,"given":"Dmitry D.","family":"Postnov","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Copenhagen  Denmark"},{"name":"Neurophotonics Center Boston University  Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3303-1095","authenticated-orcid":false,"given":"Vladimir 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