{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:36:03Z","timestamp":1785436563301,"version":"3.56.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,1,16]],"date-time":"2020-01-16T00:00:00Z","timestamp":1579132800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>The World Health Organization recommends exclusive breastfeeding until 6\u00a0months of age as an important strategy to reduce child morbidity and mortality.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Studies have associated early weaning with the development of obesity and type 2 diabetes in adulthood.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In our model, we demonstrated that early weaning leads to increased insulin secretion in adolescent males and reduced insulin secretion in adult offspring.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Early weaned males exhibit insulin resistance in skeletal muscle.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Early weaning did not change insulin signalling in the muscle of female offspring.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Taking into account that insulin resistance is one of the primary factors for the development of type 2 diabetes mellitus, this work demonstrates the importance of breastfeeding in the fight against this disease.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Early weaning (EW) leads to short\u2010 and long\u2010term obesity and diabetes. This phenotype is also observed in experimental models, in which early\u2010weaned males exhibit abnormal insulinaemia in adulthood. However, studies regarding the effect of EW on pancreatic islets are rare. We investigated the mechanisms by which glycaemic homeostasis is altered in EW models through evaluations of insulin secretion and its signalling pathway in offspring. Lactating Wistar rats and their pups were divided into the following groups: non\u2010pharmacological EW (NPEW): mothers were wrapped with an adhesive bandage on the last 3\u00a0days of lactation; pharmacological EW (PEW): mothers received bromocriptine to inhibit prolactin (1\u00a0mg\/kg body mass\/day) on the last 3\u00a0days of lactation; and control (C): pups underwent standard weaning at PN21. Offspring of both sexes were euthanized at PN45 and PN180. At PN45, EW males showed higher insulin secretion (<jats:italic>vs<\/jats:italic>. C). At PN170, PEW males exhibited hyperglycaemia in an oral glucose tolerance test (<jats:italic>vs<\/jats:italic>. C and NPEW). At PN180, EW male offspring were heavier; however, both sexes showed higher visceral fat. Insulin secretion was lower in EW offspring of both sexes. Males from both EW groups had lower glucokinase in islets, but unexpectedly, PEW males showed higher GLUT2, than did C. EW males exhibited lower insulin signalling in muscle. EW females exhibited no changes in these parameters compared with C. We demonstrated distinct alterations in the insulin secretion of EW rats at different ages. Despite the sex dimorphism in insulin secretion in adolescence, both sexes showed impaired insulin secretion in adulthood due to EW.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp278833","type":"journal-article","created":{"date-parts":[[2019,12,12]],"date-time":"2019-12-12T01:34:32Z","timestamp":1576114472000},"page":"489-502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Early weaning induces short\u2010 and long\u2010term effects on pancreatic islets in Wistar rats of both sexes"],"prefix":"10.1113","volume":"598","author":[{"given":"Carla Bruna","family":"Pietrobon","sequence":"first","affiliation":[{"name":"Laboratory of Endocrine Physiology Biology Institute State University of Rio de Janeiro  Rio de Janeiro RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0978-7949","authenticated-orcid":false,"given":"Rosiane 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