{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:58:16Z","timestamp":1791917896828,"version":"4.3.4"},"reference-count":64,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2020,6,8]],"date-time":"2020-06-08T00:00:00Z","timestamp":1591574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01 DK124509"],"award-info":[{"award-number":["R01 DK124509"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011347","name":"University of Nevada, Reno","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2020,8]]},"abstract":"\n Key points<\/jats:title>\n \n \n \n Platelet\u2010derived growth factor receptor\u2010\u03b1 (PDGFR\u03b1) is a novel biomarker along with smooth myosin heavy chain for the pacemaker cells (previously termed \u2018atypical\u2019 smooth muscle cells) in the murine and cynomolgus monkey pelvis\u2013kidney junction.<\/jats:p>\n <\/jats:list-item>\n \n \n PDGFR\u03b1\n +<\/jats:sup>\n cells present in adventitial and urothelial layers of murine renal pelvis do not express smooth muscle myosin heavy chain (smMHC) but are in close apposition to nerve fibres.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Most c\u2010Kit\n +<\/jats:sup>\n cells in the renal pelvis are mast cells. Mast cells (CD117\n +<\/jats:sup>\n \/CD45\n +<\/jats:sup>\n ) are more abundant in the proximal renal pelvis and pelvis\u2013kidney junction regions whereas c\u2010Kit\n +<\/jats:sup>\n interstitial cells (CD117\n +<\/jats:sup>\n \/CD45\n \u2212<\/jats:sup>\n ) are found predominantly in the distal renal pelvis and ureteropelvic junction.\n <\/jats:p>\n <\/jats:list-item>\n \n \n PDGFR\u03b1\n +<\/jats:sup>\n cells are distinct from c\u2010Kit\n +<\/jats:sup>\n interstitial cells.\n <\/jats:p>\n <\/jats:list-item>\n \n \n A subset of PDGFR\u03b1\n +<\/jats:sup>\n cells express the Ca\n 2+<\/jats:sup>\n \u2010activated Cl\n \u2212<\/jats:sup>\n channel, anoctamin\u20101, across the entire renal pelvis.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Spontaneous Ca\n 2+<\/jats:sup>\n transients were observed in c\u2010Kit\n +<\/jats:sup>\n interstitial cells, smMHC\n +<\/jats:sup>\n PDGFR\u03b1 cells and smMHC\n \u2212<\/jats:sup>\n PDGFR\u03b1 cells using mice expressing genetically encoded Ca\n 2+<\/jats:sup>\n sensors.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Abstract<\/jats:title>\n \n Rhythmic contractions of the renal pelvis transport urine from the kidneys into the ureter. Specialized pacemaker cells, termed atypical smooth muscle cells (ASMCs), are thought to drive the peristaltic contractions of typical smooth muscle cells (TSMCs) in the renal pelvis. Interstitial cells (ICs) in close proximity to ASMCs and TSMCs have been described, but the role of these cells is poorly understood. The presence and distributions of platelet\u2010derived growth factor receptor\u2010\u03b1\n +<\/jats:sup>\n (PDGFR\u03b1\n +<\/jats:sup>\n ) ICs in the pelvis\u2013kidney junction (PKJ) and distal renal pelvis were evaluated. We found PDGFR\u03b1\n +<\/jats:sup>\n ICs in the adventitial layers of the pelvis, the muscle layer of the PKJ and the adventitia of the distal pelvis. PDGFR\u03b1\n +<\/jats:sup>\n ICs were distinct from c\u2010Kit\n +<\/jats:sup>\n ICs in the renal pelvis. c\u2010Kit\n +<\/jats:sup>\n ICs are a minor population of ICs in murine renal pelvis. The majority of c\u2010Kit\n +<\/jats:sup>\n cells were mast cells. PDGFR\u03b1\n +<\/jats:sup>\n cells in the PKJ co\u2010expressed smooth muscle myosin heavy chain (smMHC) and several other smooth muscle gene transcripts, indicating these cells are ASMCs, and PDGFR\u03b1 is a novel biomarker for ASMCs. PDGFR\u03b1\n +<\/jats:sup>\n cells also express\n Ano1<\/jats:italic>\n , which encodes a Ca\n 2+<\/jats:sup>\n \u2010activated Cl\n \u2212<\/jats:sup>\n conductance that serves as a primary pacemaker conductance in ICs of the GI tract. Spontaneous Ca\n 2+<\/jats:sup>\n transients were observed in c\u2010Kit\n +<\/jats:sup>\n ICs, smMHC\n +<\/jats:sup>\n PDGFR\u03b1 cells and smMHC\n \u2212<\/jats:sup>\n PDGFR\u03b1 cells using genetically encoded Ca\n 2+<\/jats:sup>\n sensors. A reporter strain of mice with enhanced green fluorescent protein driven by the endogenous promotor for\n Pdgfra<\/jats:italic>\n was shown to be a powerful new tool for isolating and characterizing the phenotype and functions of these cells in the renal pelvis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp278888","type":"journal-article","created":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T06:56:47Z","timestamp":1589612207000},"page":"3283-3307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Identification and classification of interstitial cells in the mouse renal pelvis"],"prefix":"10.1113","volume":"598","author":[{"given":"Nathan","family":"Grainger","sequence":"first","affiliation":[{"name":"Department of Physiology & Cell Biology University of Nevada Reno School of Medicine Reno NV 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