{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:41:03Z","timestamp":1787863263496,"version":"build-2784847793"},"reference-count":69,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2020,9,16]],"date-time":"2020-09-16T00:00:00Z","timestamp":1600214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["1482\/3"],"award-info":[{"award-number":["1482\/3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["MA\u20105202\/1\u20101"],"award-info":[{"award-number":["MA\u20105202\/1\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["1\u20132"],"award-info":[{"award-number":["1\u20132"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\"><jats:list-item><jats:p>Neurons of the enteric submucous plexus are challenged by osmolar fluctuations during digestion and absorption of nutrients.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Central neurons are very sensitive to changes in osmolality but knowledge on that issue related to enteric neurons is sparse.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>The present study focuses on investigation of osmosensitivity of submucosal neurons including potential molecular mediating mechanisms.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>Results show that submucosal neurons respond to hypoosmolar stimuli with increased activity which is partially mediated by the transient receptor potential vanilloid 4 channel.<\/jats:p><\/jats:list-item><jats:list-item><jats:p>We provided important information on osmosensitive properties of enteric neurons. These data are fundamental to better explain the nerve\u2010mediated control of the gastrointestinal functions during physiological and pathophysiological (diarrhoea) conditions.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Enteric neurons are located inside the gut wall, where they are confronted with changes in osmolality during (inter\u2010) digestive periods. In particular, neurons of the submucous plexus (SMP), located between epithelial cells and blood vessels may sense and respond to osmotic shifts. The present study was conducted to investigate osmosensitivity of enteric submucosal neurons and the potential role of the transient receptor potential vanilloid 4 channel (TRPV4) as a mediator of enteric neuronal osmosensitivity. Therefore, freshly dissected submucosal preparations from guinea pig colon were investigated for osmosensitivity using voltage\u2010sensitive dye and Ca<jats:sup>2+<\/jats:sup>imaging. Acute hypoosmolar stimuli (final osmolality reached at ganglia of 94, 144 and 194\u00a0mOsm kg<jats:sup>\u20101<\/jats:sup>) were applied to single ganglia using a local perfusion system. Expression of TRPV4 in the SMP was quantified using qRT\u2010PCR, and GSK1016790A and HC\u2010067047 were used to activate or block the receptor, respectively, revealing its relevance in enteric osmosensitivity. On average, 11.0 [7.0\/17.0] % of submucosal neurons per ganglion responded to the hypoosmolar stimulus. The Ca<jats:sup>2+<\/jats:sup>imaging experiments showed that glia responded to the hypoosmolar stimulus, but with a delay in comparison with neurons. mRNA expression of TRPV4 could be shown in the SMP and blockade of the receptor by HC\u2010067047 significantly decreased the number of responding neurons (0.0 [0.0\/6.3] %) while the TRPV4 agonist GSK1016790A caused action potential discharge in a subpopulation of osmosensitive enteric neurons. The results of the present study provide insight into the osmosensitivity of submucosal enteric neurons and strongly indicate the involvement of TRPV4 as an osmotransducer.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jp280309","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T11:02:03Z","timestamp":1599130923000},"page":"5317-5332","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Submucosal enteric neurons of the cavine distal colon are sensitive to hypoosmolar stimuli"],"prefix":"10.1113","volume":"598","author":[{"given":"Patrick","family":"Kollmann","sequence":"first","affiliation":[{"name":"Chair of Human Biology Technical University of Munich Freising Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Elfers","sequence":"additional","affiliation":[{"name":"Institute for Physiology and Cell Biology, University of Veterinary Medicine, 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