{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:26:53Z","timestamp":1793662013214,"version":"4.5.1"},"reference-count":79,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T00:00:00Z","timestamp":1651104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acute ischaemic stroke (AIS) is a leading cause of death and disability. MicroRNAs (miRNAs) are short non\u2010coding RNAs which hold the potential to act as a novel biomarker in AIS. The majority of circulating miRNAs are actively encapsulated by extracellular vesicles (EVs) produced by many cells and organs endogenously. EVs released by mesenchymal stem cells (MSCs) have been extensively studied for their therapeutic potential. In health and disease, EVs are vital for intercellular communication, as the cargo within EVs can be exchanged between neighbouring cells or transported to distant sites. It is clear here from both current preclinical and clinical studies that AIS is associated with specific EV\u2010derived miRNAs, including those transported via MSC\u2010derived EVs. In addition, current studies provide evidence to show that modulating levels of specific EV\u2010derived miRNAs in AIS provides a novel therapeutic potential of miRNAs in the treatment of stroke. Commonalities exist in altered miRNAs across preclinical and clinical studies. Of those EV\u2010packaged miRNAs, miRNA\u2010124 was described both as an EV\u2010packaged biomarker and as a potential EV\u2010loaded therapeutic in experimental models. Alterations of miRNA\u201017 family and miRNA\u201017\u201092 cluster were identified in preclinical, clinical and MSC\u2010EV\u2010mediated neuroprotection in experimental stroke. Finally, miRNA\u201030d and \u201030a were found to mediate therapeutic effect when overexpressed from MSC and implicated as a biomarker clinically. Combined, EV\u2010derived miRNAs will further our understanding of the neuropathological processes triggered by AIS. In addition, this work will help determine the true clinical value of circulating EV\u2010packaged miRNAs as biomarkers of AIS or as novel therapeutics in this setting.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp15046-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p>","DOI":"10.1113\/jp282050","type":"journal-article","created":{"date-parts":[[2022,4,14]],"date-time":"2022-04-14T21:33:50Z","timestamp":1649972030000},"page":"4907-4921","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Extracellular vesicles and their microRNA cargo in ischaemic stroke"],"prefix":"10.1113","volume":"601","author":[{"given":"Josie L.","family":"Fullerton","sequence":"first","affiliation":[{"name":"Institute of Cardiovascular and Medical Sciences College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caitlin C.","family":"Cosgrove","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular and Medical Sciences College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca A.","family":"Rooney","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular and Medical Sciences College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6462-4109","authenticated-orcid":false,"given":"Lorraine M.","family":"Work","sequence":"additional","affiliation":[{"name":"Institute of Cardiovascular and Medical Sciences College of Medical, Veterinary and Life Sciences University of Glasgow  Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,28]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S231853"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/nan.12116"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2990.2011.01170.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124407"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1171-15.2016"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-021-00787-y"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2013.67"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-015-0429-3"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2017.00057"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-11-98"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-101512-122326"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.247452"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10040767"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13036-019-0193-0"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2017.09.058"},{"key":"e_1_2_2_17_1","first-page":"1","volume-title":"Mesenchymal stem cells in human health and diseases","author":"El\u2010Hashash A. 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