{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:26Z","timestamp":1787016206379,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,12]],"date-time":"2021-12-12T00:00:00Z","timestamp":1639267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      ATP\u2010sensitive potassium channels (K\n                      <jats:sub>ATP<\/jats:sub>\n                      channels) are hetero\u2010octameric nucleotide\u2010gated ion channels that couple cellular metabolism to excitability in various tissues. In the heart, K\n                      <jats:sub>ATP<\/jats:sub>\n                      channels are activated during ischaemia and potentially during adrenergic stimulation. In the vasculature, they are normally active at a low level, reducing vascular tone, but the ubiquitous nature of these channels leads to complex and poorly understood channelopathies as a result of gain\u2010 or loss\u2010of\u2010function mutations. Zebrafish (ZF) models of these channelopathies may provide insights to the link between molecular dysfunction and complex pathophysiology, but this requires understanding the tissue dependence of channel activity and subunit specificity. Thus far, direct analysis of ZF K\n                      <jats:sub>ATP<\/jats:sub>\n                      expression and functional properties has only been performed in pancreatic \u03b2\u2010cells. Using a comprehensive combination of genetically modified fish, electrophysiology and gene expression analysis, we demonstrate that ZF cardiac myocytes (CM) and vascular smooth muscle (VSM) express functional K\n                      <jats:sub>ATP<\/jats:sub>\n                      channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts. However, in contrast to mammalian cardiovascular K\n                      <jats:sub>ATP<\/jats:sub>\n                      channels, ZF channels are insensitive to potassium channel opener drugs (pinacidil, minoxidil) in both chambers of the heart and in VSM. The results provide a first characterization of the molecular properties of fish K\n                      <jats:sub>ATP<\/jats:sub>\n                      channels and validate the use of such genetically modified fish as models of human Cant\u00fa syndrome and ABCC9\u2010related Intellectual Disability and Myopathy syndrome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            Zebrafish cardiac myocytes (CM) and vascular smooth muscle (VSM) express functional K\n                            <jats:sub>ATP<\/jats:sub>\n                            channels of similar subunit composition, structure and metabolic sensitivity to their mammalian counterparts.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            In contrast to mammalian cardiovascular K\n                            <jats:sub>ATP<\/jats:sub>\n                            channels, zebrafish channels are insensitive to potassium channel opener drugs (pinacidil, minoxidil) in both chambers of the heart and in VSM.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            We provide a first characterization of the molecular properties of fish K\n                            <jats:sub>ATP<\/jats:sub>\n                            channels and validate the use of such genetically modified fish as models of human Cant\u00fa syndrome and ABCC9\u2010related Intellectual Disability and Myopathy syndrome.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp282157","type":"journal-article","created":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T07:20:16Z","timestamp":1637824816000},"page":"299-312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["ATP\u2010sensitive potassium channels in zebrafish cardiac and vascular smooth muscle"],"prefix":"10.1113","volume":"600","author":[{"given":"Soma S.","family":"Singareddy","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Physiology and Center for the Investigation of Membrane Excitability Diseases Washington University in St Louis  St Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen I.","family":"Roessler","sequence":"additional","affiliation":[{"name":"Department of Genetics Center for Molecular Medicine University 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