{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:08:58Z","timestamp":1792706938425,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T00:00:00Z","timestamp":1633910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"\n Abstract<\/jats:title>\n \n Boys with Duchenne muscular dystrophy (DMD) experience a progressive loss of functional muscle mass, with fibrosis and lipid accumulation. Accurate evaluation of whole\u2010body functional muscle mass (MM) in DMD patients has not previously been possible and the rate of synthesis of muscle proteins remains unexplored. We used non\u2010invasive, stable isotope\u2010based methods from plasma and urine to measure the fractional rate of muscle protein synthesis (FSR) functional muscle mass (MM), and fat free mass (FFM) in 10 DMD (6\u201317\u00a0years) and 9 age\u2010matched healthy subjects. An oral dose of D\n 3<\/jats:sub>\n creatine in 70%\n 2<\/jats:sup>\n H\n 2<\/jats:sub>\n O was administered to determine MM and FFM followed by daily 70%\n 2<\/jats:sup>\n H\n 2<\/jats:sub>\n O to measure protein FSR. Functional MM was profoundly reduced in DMD subjects compared to controls (17%\n vs<\/jats:italic>\n . 41% of body weight,\n P<\/jats:italic>\n \u00a0<\u00a00.0001), particularly in older, non\u2010ambulant patients in whom functional MM was extraordinarily low (<13% body weight). We explored the urine proteome to measure FSR of skeletal muscle\u2010derived proteins. Titin, myosin light chain and gelsolin FSRs were substantially lower in DMD subjects compared to controls (27%, 11% and 40% of control, respectively,\n P<\/jats:italic>\n \u00a0<\u00a00.0001) and were strongly correlated. There were no differences in muscle\u2010derived sarcoplasmic proteins FSRs (creatine kinase M\u2010type and carbonic anhydrase\u20103) measured in plasma. These data demonstrate that both functional MM, body composition and muscle protein synthesis rates can be quantified non\u2010invasively and are markedly different between DMD and control subjects and suggest that the rate of contractile but not sarcoplasmic protein synthesis is affected by a lack of dystrophin.\n <\/jats:p>\n <\/jats:sec>\n \n Key points<\/jats:title>\n \n \n \n Duchenne muscular dystrophy (DMD) results in a progressive loss of functional skeletal muscle but total body functional muscle mass or rates of muscle protein synthesis have not previously been assessed in these patients.<\/jats:p>\n <\/jats:list-item>\n \n \n D\n 3<\/jats:sub>\n \u2010creatine dilution was used to measure total functional muscle mass and oral\n 2<\/jats:sup>\n H\n 2<\/jats:sub>\n O was used to examine the rates of muscle protein synthesis non\u2010invasively in boys with DMD and healthy controls using urine samples.\n <\/jats:p>\n <\/jats:list-item>\n \n Muscle mass was profoundly lower in DMD compared to control subjects, particularly in older, non\u2010ambulant patients.<\/jats:p>\n <\/jats:list-item>\n \n The rates of contractile protein synthesis but not sarcoplasmic proteins were substantially lower in DMD.<\/jats:p>\n <\/jats:list-item>\n \n These results may provide non\u2010invasive biomarkers for disease progression and therapeutic efficacy in DMD and other neuromuscular diseases.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp282227","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T03:24:07Z","timestamp":1632713047000},"page":"5215-5227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Profoundly lower muscle mass and rate of contractile protein synthesis in boys with Duchenne muscular dystrophy"],"prefix":"10.1113","volume":"599","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6757-1471","authenticated-orcid":false,"given":"William J.","family":"Evans","sequence":"first","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"},{"name":"Department of Medicine Duke Medical Center Durham NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahalakshmi","family":"Shankaran","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward C.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Duke Medical Center Durham NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Morris","sequence":"additional","affiliation":[{"name":"Solid Biosciences, Inc Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7430-3181","authenticated-orcid":false,"given":"Edna","family":"Nyangau","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alec","family":"Bizieff","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcy","family":"Matthews","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hussein","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Hellerstein","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences and Toxicology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M110.000992"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2011.06.750"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000009012"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153461"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.3233\/JND-140066"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gly129"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-021-00386-2"},{"key":"e_1_2_5_9_1","first-page":"151","article-title":"Multi\u2010slice MRI reveals heterogeneity in disease distribution along the length of muscle in Duchenne muscular dystrophy","volume":"36","author":"Chrzanowski SM","year":"2017","journal-title":"Acta Myol"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00045.2014"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28860"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-958463"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41390-020-01122-w"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0241722"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192571199"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48548-9"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12014-016-9109-x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1962.sp007000"},{"key":"e_1_2_5_19_1","first-page":"184","article-title":"The role of fibrosis in Duchenne muscular dystrophy","volume":"31","author":"Klingler W","year":"2012","journal-title":"Acta Myol"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.186.2.8421754"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/41.2.363"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201300072"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23982"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-017-9478-4"},{"key":"e_1_2_5_25_1","first-page":"4263","article-title":"Bi\u2010allelic mutations in MYL1 cause a severe congenital myopathy","volume":"27","author":"Ravenscroft G","year":"2018","journal-title":"Hum Mol Genet"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/296165a0"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2017.05.009"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12278"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI79639"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21212"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13539-013-0110-1"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00122.2012"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.3233\/JND-190454"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199909)22:9<1228::AID-MUS9>3.0.CO;2-6"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00711948"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.26081"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP282227","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/JP282227","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP282227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T09:34:58Z","timestamp":1693215298000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP282227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,11]]},"references-count":35,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1113\/JP282227"],"URL":"https:\/\/doi.org\/10.1113\/jp282227","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,11]]},"assertion":[{"value":"2021-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}