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In a cross\u2010species study using small and large animals, we comprehensively tested the agreement and validity of multiple single\u2010beat surrogate metrics of LV contractility against the field\u2010standard metrics derived from inferior vena cava occlusion (IVCO). Fifty\u2010six rats, 27 minipigs and 11 conscious dogs underwent LV and arterial catheterization and were assessed for a range of single\u2010beat metrics of LV contractility. All single\u2010beat metrics were tested for the various underlying assumptions required to be considered a valid metric of cardiac contractility, including load\u2010independency, sensitivity to inotropic stimulation, and ability to diagnose contractile dysfunction in cardiac disease. Of all examined single\u2010beat metrics, only LV maximal pressure normalized to end\u2010diastolic volume (EDV), end\u2010systolic pressure normalized to EDV, and the maximal rate of rise of the LV pressure normalized to EDV showed a moderate\u2010to\u2010excellent agreement with their IVCO\u2010derived reference measure and met all the underlying assumptions required to be considered as a valid cardiac contractile metric in both rodents and large\u2010animal models. Our findings demonstrate that single\u2010beat metrics can be used as a valid, reliable method to quantify cardiac contractile function in basic\/preclinical experiments utilizing small\u2010 and large\u2010animal models\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp15283-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Validating and comparing indices of cardiac contractility that avoid caval occlusion would offer considerable advantages for the field of cardiovascular physiology.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We comprehensively test the underlying assumptions of multiple single\u2010beat indices of cardiac contractility in rodents and translate these findings to pigs and conscious dogs.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We show that when performing caval occlusion is unfeasible, single\u2010beat metrics can be utilized to accurately quantify cardiac inotropic function in basic and preclinical research employing various small and large animal species.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We report that maximal left\u2010ventricular\u00a0(LV)\u2010pressure normalized to end\u2010diastolic volume (EDV), LV end\u2010systolic pressure normalized to EDV and the maximal rate of rise of the LV pressure waveform normalized to EDV are the best three single\u2010beat metrics to measure cardiac inotropic function in both small\u2010 and large\u2010animal models.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp283319","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T16:44:41Z","timestamp":1663605881000},"page":"4779-4806","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A cross\u2010species validation of single\u2010beat metrics of cardiac contractility"],"prefix":"10.1113","volume":"600","author":[{"given":"Mehdi","family":"Ahmadian","sequence":"first","affiliation":[{"name":"School of Kinesiology Faculty of Education University of British Columbia  Vancouver BC Canada"},{"name":"International Collaboration on Repair Discoveries University of British Columbia  Vancouver BC Canada"},{"name":"Centre for Chronic Disease Prevention and Management University of British Columbia  Kelowna BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1571-9168","authenticated-orcid":false,"given":"Alexandra M.","family":"Williams","sequence":"additional","affiliation":[{"name":"International Collaboration on Repair Discoveries University of British Columbia  Vancouver BC Canada"},{"name":"Department of Cellular and Physiological Sciences Faculty of Medicine University of British Columbia  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Mannozzi","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Medicine Wayne State University  Detroit MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5930-8040","authenticated-orcid":false,"given":"Filip","family":"Konecny","sequence":"additional","affiliation":[{"name":"Transonic Scisense Inc.  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