{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:42:42Z","timestamp":1788385362387,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T00:00:00Z","timestamp":1719187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021720","name":"Olav Thon Stiftelsen","doi-asserted-by":"publisher","award":["16\u20101606"],"award-info":[{"award-number":["16\u20101606"]}],"id":[{"id":"10.13039\/501100021720","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Aquaporin\u20104 (AQP4) is the main water channel in brain and is enriched in perivascular astrocyte processes abutting brain microvessels. There is a rich literature on the role of AQP4 in experimental stroke. While its role in oedema formation following middle cerebral artery occlusion (MCAO) has been studied extensively, its specific impact on infarct volume remains unclear. This study investigated the effects of total and partial AQP4 deletion on infarct volume in mice subjected to distal medial cerebral artery (dMCAO) occlusion. Compared to MCAO, this model induces smaller infarcts confined to neocortex, and less oedema. We show that AQP4 deletion significantly reduced infarct volume as assessed 1\u00a0week after dMCAO, suggesting that the role of AQP4 in stroke goes beyond its effect on oedema formation and dissolution. The reduction in infarct volume was associated with increased astrocyte reactivity in the peri\u2010infarct areas. No significant differences were observed in the number of microglia among the genotypes. These findings provide new insights in the role of AQP4 in ischaemic injury indicating that AQP4 affects both infarct volume and astrocyte reactivity in the peri\u2010infarct zone.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16159-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Aquaporin\u20104 (AQP4) is the main water channel in brain and is enriched in perivascular astrocyte processes abutting microvessels.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>A rich literature exists on the role of AQP4 in oedema formation following middle cerebral artery occlusion (MCAO).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We investigated the effects of total and partial AQP4 deletion on infarct volume in mice subjected to distal medial cerebral artery occlusion (dMCAO), a model inducing smaller infarcts confined to neocortex and less oedema compared to MCAO.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>AQP4 deletion significantly reduced infarct volume 1\u00a0week after dMCAO, suggesting a broader role for AQP4 in stroke beyond oedema formation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The reduction in infarct volume was associated with increased astrocyte reactivity in the peri\u2010infarct areas, while no significant differences were observed in the number of microglia among the genotypes.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings provide new insights into the role of AQP4 in stroke, indicating that AQP4 affects both infarct volume and astrocyte reactivity in the peri\u2010infarct zone.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp284099","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T16:50:47Z","timestamp":1719247847000},"page":"3151-3168","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Aquaporin\u20104 deletion leads to reduced infarct volume and increased peri\u2010infarct astrocyte reactivity in a mouse model of cortical stroke"],"prefix":"10.1113","volume":"602","author":[{"given":"Nadia","family":"Skauli","sequence":"first","affiliation":[{"name":"Division of Anatomy, Department of Molecular Medicine, Institute of Basic Medical Sciences University of Oslo  Blindern Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Negar","family":"Zohoorian","sequence":"additional","affiliation":[{"name":"Division of Anatomy, Department of Molecular Medicine, Institute of Basic Medical Sciences 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